Esg1, expressed exclusively in preimplantation embryos, germline, and embryonic stem cells, is a putative RNA-binding protein with broad RNA targets

Tetsuya S. Tanaka, Isabel Lopez De Silanes, Lioudmila V. Sharova, Hidenori Akutsu, Toshiyuki Yoshikawa, Hisayuki Amano, Shinya Yamanaka, Myriam Gorospe, Minoru Ko

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

In our earlier attempt to identify genes involved in the maintenance of cellular pluripotency, we found that KH-domain protein Embryonal stem cell-specific gene 1 (Esg1) showed similar expression patterns to those of Oct3/4 (Pou5f1), whereas the forced repression of Oct3/4 in mouse embryonic stem cells immediately downregulated the expression of Esg1. Here we further confirm this overlap by in situ hybridization and immunohistochemical analyses. Both Esg1 transcript and protein exist in the egg and preimplantation embryos. At embryonic day 3.5, blastocyst stage, however, ESG1 protein was more abundant in the inner cell mass (ICM) than in trophectoderm (TE), whereas Esg1 transcript was detected in both the ICM and the TE, particularly in the polar trophectoderm. The presence of an RNA-binding KH-domain in ESG1 led us to search for and identify 902 target transcripts by microarray analysis of immunoprecipitated ESG1 complex. Interaction of 20 target mRNA with ESG1, including Cdc25a, Cdc42, Ezh2, Nfyc and Nr5a2. was further validated by reverse transcriptase-polymerase chain reaction of the immunoprecipitation material, supporting the notion that ESG1 is an RNA-binding protein which associates with specific target transcripts.

Original languageEnglish
Pages (from-to)381-390
Number of pages10
JournalDevelopment Growth and Differentiation
Volume48
Issue number6
DOIs
Publication statusPublished - 2006 Aug
Externally publishedYes

Fingerprint

RNA-Binding Proteins
Blastocyst
Embryonic Stem Cells
Stem Cells
RNA
Genes
Microarray Analysis
Reverse Transcriptase Polymerase Chain Reaction
Immunoprecipitation
In Situ Hybridization
Ovum
Proteins
Down-Regulation
Maintenance
Messenger RNA

Keywords

  • Cellular pluripotency
  • Esg1
  • Immunoprecipitation-microarray
  • RNA-binding protein

ASJC Scopus subject areas

  • Anatomy
  • Developmental Biology
  • Cell Biology

Cite this

Esg1, expressed exclusively in preimplantation embryos, germline, and embryonic stem cells, is a putative RNA-binding protein with broad RNA targets. / Tanaka, Tetsuya S.; De Silanes, Isabel Lopez; Sharova, Lioudmila V.; Akutsu, Hidenori; Yoshikawa, Toshiyuki; Amano, Hisayuki; Yamanaka, Shinya; Gorospe, Myriam; Ko, Minoru.

In: Development Growth and Differentiation, Vol. 48, No. 6, 08.2006, p. 381-390.

Research output: Contribution to journalArticle

Tanaka, Tetsuya S. ; De Silanes, Isabel Lopez ; Sharova, Lioudmila V. ; Akutsu, Hidenori ; Yoshikawa, Toshiyuki ; Amano, Hisayuki ; Yamanaka, Shinya ; Gorospe, Myriam ; Ko, Minoru. / Esg1, expressed exclusively in preimplantation embryos, germline, and embryonic stem cells, is a putative RNA-binding protein with broad RNA targets. In: Development Growth and Differentiation. 2006 ; Vol. 48, No. 6. pp. 381-390.
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