Essential contribution of IRF3 to intestinal homeostasis and microbiota-mediated Tslp gene induction

Hideo Negishi, Shoji Miki, Hana Sarashina, Naoko Taguchi-Atarashi, Akira Nakajima, Kosuke Matsuki, Nobuyasu Endo, Hideyuki Yanai, Junko Nishio, Kenya Honda, Tadatsugu Taniguchi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The large intestinal epithelial cells and immune cells are exposed to a variety of molecules derived from commensal microbiota that can activate innate receptors, such as Toll-like receptors (TLRs) and retinoic acid-inducible gene-I-like receptors (RLRs). Although the activation of these receptors is known to be critical for homeostasis of the large intestine, the underlying gene regulatory mechanisms are not well understood. Here, we show that IFN regulatory factor (IRF)3 is critical for the suppression of dextran sulfate sodium-induced colitis. IRF3-deficient mice exhibited lethal defects in the inflammatory and recovery phases of the colitis, accompanied by marked defects in the gene induction for thymic stromal lymphopoietin (TSLP), a cytokine knownto be essential for protection of the large intestine. We further provide evidence that DNA and RNA of the large intestinal contents are critical for Tslp gene induction via IRF3 activation by cytosolic nucleic acid receptors. We also demonstrate that IRF3 indeed activates the gene promoter of Tslp via IRF-binding sequences. This newly identified intestinal gene regulatory mechanism, wherein IRF3 activated by microbiota-derived nucleic acids plays a critical role in intestinal homeostasis, may have clinical implication in colonic inflammatory disorders.

Original languageEnglish
Pages (from-to)21016-21021
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number51
DOIs
Publication statusPublished - 2012 Dec 18
Externally publishedYes

Fingerprint

Homeostasis
Microbiota
Large Intestine
Colitis
Regulator Genes
Nucleic Acids
Genes
Interferon Regulatory Factor-3
Dextran Sulfate
Gastrointestinal Contents
Toll-Like Receptors
Tretinoin
Epithelial Cells
RNA
Cytokines
Gastrointestinal Microbiome
DNA

Keywords

  • IL33
  • Pattern recognition receptor

ASJC Scopus subject areas

  • General

Cite this

Essential contribution of IRF3 to intestinal homeostasis and microbiota-mediated Tslp gene induction. / Negishi, Hideo; Miki, Shoji; Sarashina, Hana; Taguchi-Atarashi, Naoko; Nakajima, Akira; Matsuki, Kosuke; Endo, Nobuyasu; Yanai, Hideyuki; Nishio, Junko; Honda, Kenya; Taniguchi, Tadatsugu.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 51, 18.12.2012, p. 21016-21021.

Research output: Contribution to journalArticle

Negishi, H, Miki, S, Sarashina, H, Taguchi-Atarashi, N, Nakajima, A, Matsuki, K, Endo, N, Yanai, H, Nishio, J, Honda, K & Taniguchi, T 2012, 'Essential contribution of IRF3 to intestinal homeostasis and microbiota-mediated Tslp gene induction', Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 51, pp. 21016-21021. https://doi.org/10.1073/pnas.1219482110
Negishi, Hideo ; Miki, Shoji ; Sarashina, Hana ; Taguchi-Atarashi, Naoko ; Nakajima, Akira ; Matsuki, Kosuke ; Endo, Nobuyasu ; Yanai, Hideyuki ; Nishio, Junko ; Honda, Kenya ; Taniguchi, Tadatsugu. / Essential contribution of IRF3 to intestinal homeostasis and microbiota-mediated Tslp gene induction. In: Proceedings of the National Academy of Sciences of the United States of America. 2012 ; Vol. 109, No. 51. pp. 21016-21021.
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