Essential roles of Windei and nuclear monoubiquitination of Eggless/SETDB1 in transposon silencing

Ken Osumi, Kaoru Sato, Kensaku Murano, Haruhiko Siomi, Mikiko C. Siomi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Eggless/SETDB1 (Egg), the only essential histone methyltransferase (HMT) in Drosophila, plays a role in gene repression, including piRNA-mediated transposon silencing in the ovaries. Previous studies suggested that Egg is post-translationally modified and showed that Windei (Wde) regulates Egg nuclear localization through protein–protein interaction. Monoubiquitination of mammalian SETDB1 is necessary for the HMT activity. Here, using cultured ovarian somatic cells, we show that Egg is monoubiquitinated and phosphorylated but that only monoubiquitination is required for piRNA-mediated transposon repression. Egg monoubiquitination occurs in the nucleus. Egg has its own nuclear localization signal, and the nuclear import of Egg is Wde-independent. Wde recruits Egg to the chromatin at target gene silencing loci, but their interaction is monoubiquitin-independent. The abundance of nuclear Egg is governed by that of nuclear Wde. These results illuminate essential roles of nuclear monoubiquitination of Egg and the role of Wde in piRNA-mediated transposon repression.

Original languageEnglish
Article numbere48296
JournalEMBO Reports
DOIs
Publication statusAccepted/In press - 2019 Jan 1

Fingerprint

Small Interfering RNA
Genes
Nuclear Localization Signals
Cell Nucleus Active Transport
Gene Silencing
Drosophila
Chromatin
Ovary
histone methyltransferase

Keywords

  • Eggless
  • monoubiquitination
  • piRNA
  • SETDB1
  • Windei

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Essential roles of Windei and nuclear monoubiquitination of Eggless/SETDB1 in transposon silencing. / Osumi, Ken; Sato, Kaoru; Murano, Kensaku; Siomi, Haruhiko; Siomi, Mikiko C.

In: EMBO Reports, 01.01.2019.

Research output: Contribution to journalArticle

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