Establishing an induced pluripotent stem cell line from neonatal common marmoset fibroblasts by an all-in-one episomal vector approach

Sho Yoshimatsu, Emi Qian, Tsukika Sato, Masafumi Yamamoto, Mitsuru Ishikawa, Hideyuki Okano

Research output: Contribution to journalArticlepeer-review

Abstract

Epstein-Barr virus (EBV)-based episomal vector system enables persistent transgene expression, which is advantageous for efficient derivation of transgene-free induced pluripotent stem cells (iPSCs) without viral transduction. Here, we report establishment of an iPSC line from somatic fibroblasts of a neonatal common marmoset monkey (marmoset; Callithrix jacchus) using an all-in-one episomal vector that we newly developed. The established iPSC line, named NM-iPS, showed standard characteristics of pluripotency such as pluripotency-related marker expression, three germ layer differentiation, and normal karyotype (2n = 46). The novel iPSC line would be a useful resource for stem cell research using non-human primates.

Original languageEnglish
Article number102380
JournalStem Cell Research
Volume53
DOIs
Publication statusPublished - 2021 May

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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