Establishment and characterization of a human chorionic gonadotropin (hCG) producing cell line (RTSG) from an ovarian epithelial cancer

Y. Udagawa, S. Nozawa, K. Chin, M. Sakayori, M. Mikami, K. Ohta, K. Tsukazaki, K. Kiguchi, R. Iizuka

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A new cell line designated RTSG established in vitro from the pleural effusion of a patient with metastatic ovarian epithelial cancer has been subcultured 46 times for more than 2 years. The cells grew in a monolayered sheet, showing a tendency to pile up, with the population doubling in 48 hrs. Electron-microscopically, desmosomes were characteristically observed, suggesting the cells were of epithelial origin. Chromosomal analysis revealed aneuploidy with a tetraploid mode. The heterotransplanted tumors in nude mice were histopathologically classified as a poorly differentiated adenocarcinoma, whereas the original tumor consisted mainly of mucinous and serous cystadenocarcinoma and only partly of poorly differentiated adenocarcinoma. The cells secreted hCG (38.8 mIU/day/10(6) cells) and beta-hCG (6.1 ng/day/10(6) cells) in spent medium. Immunocytologic +-and-histochemical staining for tumor markers of the original tumor, the cultured cells and the transplanted tumors also revealed the localization of not only hCG and beta-hCG but also CA19-9 and CA-125 whose values had been elevated in the preoperative serum (hCG: 10 mIU/ml, CA19-9: 6,400 U/ml, CA-125: 225 U/ml). Results of PAS, Alcian-blue and Mucicarmine strains indicated that most of the PAS-positive substances in the cultured cells and the transplanted tumors were consistent with glycogen while the original tumor mainly contained mucin except for the lesion of poorly differentiated adenocarcinoma with glycogen. These results suggested that the cultured cells might originate from poorly differentiated adenocarcinoma cells in the original tumor.

Original languageEnglish
Pages (from-to)70-75
Number of pages6
JournalHuman cell : official journal of Human Cell Research Society
Volume3
Issue number1
Publication statusPublished - 1990 Mar

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Chorionic Gonadotropin
Adenocarcinoma
Cell Line
Cultured Tumor Cells
Neoplasms
Glycogen
Serous Cystadenocarcinoma
Mucinous Cystadenocarcinoma
Alcian Blue
Desmosomes
Tetraploidy
Aneuploidy
Mucins
Pleural Effusion
Tumor Biomarkers
Heterografts
Nude Mice
Cultured Cells
Epithelial Cells
Ovarian epithelial cancer

ASJC Scopus subject areas

  • Medicine(all)

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Establishment and characterization of a human chorionic gonadotropin (hCG) producing cell line (RTSG) from an ovarian epithelial cancer. / Udagawa, Y.; Nozawa, S.; Chin, K.; Sakayori, M.; Mikami, M.; Ohta, K.; Tsukazaki, K.; Kiguchi, K.; Iizuka, R.

In: Human cell : official journal of Human Cell Research Society, Vol. 3, No. 1, 03.1990, p. 70-75.

Research output: Contribution to journalArticle

Udagawa, Y, Nozawa, S, Chin, K, Sakayori, M, Mikami, M, Ohta, K, Tsukazaki, K, Kiguchi, K & Iizuka, R 1990, 'Establishment and characterization of a human chorionic gonadotropin (hCG) producing cell line (RTSG) from an ovarian epithelial cancer', Human cell : official journal of Human Cell Research Society, vol. 3, no. 1, pp. 70-75.
Udagawa, Y. ; Nozawa, S. ; Chin, K. ; Sakayori, M. ; Mikami, M. ; Ohta, K. ; Tsukazaki, K. ; Kiguchi, K. ; Iizuka, R. / Establishment and characterization of a human chorionic gonadotropin (hCG) producing cell line (RTSG) from an ovarian epithelial cancer. In: Human cell : official journal of Human Cell Research Society. 1990 ; Vol. 3, No. 1. pp. 70-75.
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