Establishment and characterization of novel cell lines and xenografts from patients with gastrointestinal stromal tumors

Kazumasa Fukuda, Yoshirou Saikawa, Hiroyuki Sako, Yumi Yoshimura, Tsunehiro Takahashi, Norihito Wada, Hirohumi Kawakubo, Hiroya Takeuchi, Tai Ohmori, Yuko Kitagawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

At present, no suitable GIST model exists for the analysis of drug resistance or metastasis using established human gastrointestinal stromal tumor (GIST) cell lines or xenografts even though the molecular mechanisms of drug resistance, progression and metastasis require clarification. The aim of this study was to establish and characterize human GIST cell lines and xenografts that can be used for evaluating drug resistance or various new molecularly targeted therapies. GIST tissues from patients were cultured and implanted under the skin of NOG (NOD/Shi-scid, IL-2Rrnu) mice. Two new cell lines (GK1C and GK3C) and three xenografts (GK1X, GK2X and GK3X) were generated from these clinical samples. The established GIST cell lines and xenografts were investigated for tumorigenesis and imatinib sensitivity. These cell lines and xenografts showed characteristic GIST morphology and exhibited KIT expression profiles similar to those of the patient samples. In addition, these GIST cell lines and xenografts were sensitive to imatinib. In conclusion, new human GIST cell lines and xenografts were established and maintained through repeated passages. These models will enable further study of combination therapies and the mechanisms of resistance, and allow testing of novel targeted monotherapies and combination therapies.

Original languageEnglish
Pages (from-to)71-78
Number of pages8
JournalOncology reports
Volume30
Issue number1
DOIs
Publication statusPublished - 2013 Jul

Keywords

  • GIST
  • Imatinib
  • PDGFRA
  • c-KIT

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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