Establishment and characterization of novel patient-derived cell lines from giant cell tumor of bone

Yuki Yoshimatsu, Rei Noguchi, Ryuto Tsuchiya, Takuya Ono, Yooksil Sin, Sei Akane, Jun Sugaya, Tomoaki Mori, Suguru Fukushima, Akihiko Yoshida, Akira Kawai, Tadashi Kondo

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Giant cell tumor of bone (GCTB) is a locally aggressive and rarely metastasizing tumor. GCTB is characterized by the presence of unique giant cells and a recurrent mutation in the histone tail of the histone variant H3.3, which is encoded by H3F3A on chromosome 1. GCTB accounts for ~ 5% of primary bone tumors. Although GCTB exhibits an indolent course, it has the potential to develop aggressive behaviors associated with local recurrence and distant metastasis. Currently, complete surgical resection is the only curative treatment, and novel therapeutic strategies are required. Patient-derived cancer cell lines are critical tools for basic and pre-clinical research. However, only a few GCTB cell lines have been reported, and none of them are available from public cell banks. Therefore, we aimed to establish novel GCTB cell lines in the present study. Using curetted tumor tissues of GCTB, we established two cell lines and named them NCC-GCTB2-C1 and NCC-GCTB3-C1. These cells harbored a typical mutation in histones and exhibited slow but constant growth, formed spheroids, and had invasive capabilities. We demonstrated the utility of these cell lines for high-throughput drug screening using 214 anticancer agents. We concluded that NCC-GCTB2-C1 and NCC-GCTB3-C1 cell lines were useful for the in vitro study of GCTB.

Original languageEnglish
Pages (from-to)1899-1910
Number of pages12
JournalHuman Cell
Volume34
Issue number6
DOIs
Publication statusPublished - 2021 Nov
Externally publishedYes

Keywords

  • Cell lines
  • Giant cell tumor of bone
  • High-throughput screening
  • Patient-derived cancer model
  • Spheroids

ASJC Scopus subject areas

  • Cell Biology
  • Cancer Research

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