Establishment and Long-Term Culture of Organoids Derived from Human Biliary Tract Carcinoma

Yoshimasa Saito, Toshihide Muramatsu, Hidetsugu Saito

Research output: Contribution to journalArticlepeer-review

Abstract

This protocol is a procedure for establishment and culture of cancer and non-cancer organoids using tissues from biliary tract carcinoma (BTC) patients. These BTC organoids can be used for various biological analyses and drug screening. One challenge in establishing and culturing BTC organoids is non-cancer cells contaminating surgically resected tumor tissues form organoids concurrently with cancer organoids. Careful validation that the established organoids are cancer-derived is important. For complete details on the use and generation of this protocol, please refer to Saito et al. (2019) in the journal Cell Reports.

Original languageEnglish
Article number100009
JournalSTAR Protocols
Volume1
Issue number1
DOIs
Publication statusPublished - 2020 Jun 19

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)

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