Establishment of a continuous culture system for entamoeba muris and analysis of the small subunit rrna gene

S. Kobayashi, J. Suzuki, T. Takeuchi

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4 Citations (Scopus)

Abstract

We established a culture system for Entamoeba muris (MG-EM-01 strain isolated from a Mongolian gerbil) using a modified Balamuth's egg yolk infusion medium supplemented with 4 % adult bovine serum and Baderoides fragilis cocultured with Escherichia coli. Further, encystation was observed in the culture medium. The morphological characteristics of E. muris are similar to those of Entamoeba coli (E. coli); moreover, the malic isoenzyme electrophoretic band, which shows species-specific electrophoretic mobility, of E. muris had almost the same mobility as that observed with the malic isoenzyme electrophorectic band of E. coli (UZG-EC-01 strain isolated from a gorilla). We determined the small subunit rRNA (SSU-rRNA) gene sequence of the MG-EM-01 strain, and this sequence was observed to show 82.7 % homology with that of the UZG-EC-01 strain. Further, the resultant phylogenetic tree for molecular taxonomy based on the SSU-rRNA genes of the 21 strains of the intestinal parasitic amoeba species indicated that the MG-EM-01 strain was most closely related to E. coli.

Original languageEnglish
Pages (from-to)135-139
Number of pages5
JournalParasite
Volume16
Issue number2
DOIs
Publication statusPublished - 2009 Jun

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Keywords

  • Balamuth's egg yolk infusion medium
  • Dixenic culture
  • Entamoeba coli
  • Entamoeba muris
  • Phylogenetic analysis
  • SSU-rRNA gene

ASJC Scopus subject areas

  • Parasitology
  • Animal Science and Zoology
  • veterinary (miscalleneous)
  • Insect Science
  • Infectious Diseases

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