Establishment of a human cell line (HCC-T) from a patient with hepatoma bearing no evidence of hepatitis B or a virus infection

Hidetsugu Saito, T. Morizane, T. Watanabe, T. Kagawa, N. Iwabuchi, N. Kumagai, Y. Inagaki, K. Tsuchimoto, M. Tsuchiya

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Abstract

A human hepatoma cell line, designated HCC-T, was established. The tumor was surgically obtained from a Japanese male patient with hepatocellular carcinoma (HCC) arising in a cirrhotic liver that had supposedly developed from nonAnonB (NANB) chronic hepatitis. HCC-T exhibited a typical morphology of epithelial cells in culture. Population doubling time was 24 hours and HCC-T cells had characteristics of malignant cells demonstrated by the ability to grow in a soft agar medium and transplantability to nude mice. The histologic condition of the tumor transplanted to a nude mouse showed similarity to the original tumor. A chromosome analysis showed that there were ten identifiable marker chromosomes and sex chromosomes with its modal number of 64. Alpha-fetoprotein (AFP) production was demonstrated by direct immunofluorescence study, but albumin or hepatitis B surface antigen were not detectable. The integration of hepatitis B viral DNA was not demonstrable in the genome of HCC-T cells or the original hepatoma.

Original languageEnglish
Pages (from-to)1054-1060
Number of pages7
JournalCancer
Volume64
Issue number5
Publication statusPublished - 1989

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Virus Diseases
Hepatitis B
Hepatocellular Carcinoma
Cell Line
Nude Mice
T-Lymphocytes
Neoplasms
Direct Fluorescent Antibody Technique
Sex Chromosomes
Viral DNA
alpha-Fetoproteins
Chronic Hepatitis
Hepatitis B Surface Antigens
Genetic Markers
Agar
Albumins
Cell Culture Techniques
Chromosomes
Epithelial Cells
Genome

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Saito, H., Morizane, T., Watanabe, T., Kagawa, T., Iwabuchi, N., Kumagai, N., ... Tsuchiya, M. (1989). Establishment of a human cell line (HCC-T) from a patient with hepatoma bearing no evidence of hepatitis B or a virus infection. Cancer, 64(5), 1054-1060.

Establishment of a human cell line (HCC-T) from a patient with hepatoma bearing no evidence of hepatitis B or a virus infection. / Saito, Hidetsugu; Morizane, T.; Watanabe, T.; Kagawa, T.; Iwabuchi, N.; Kumagai, N.; Inagaki, Y.; Tsuchimoto, K.; Tsuchiya, M.

In: Cancer, Vol. 64, No. 5, 1989, p. 1054-1060.

Research output: Contribution to journalArticle

Saito, H, Morizane, T, Watanabe, T, Kagawa, T, Iwabuchi, N, Kumagai, N, Inagaki, Y, Tsuchimoto, K & Tsuchiya, M 1989, 'Establishment of a human cell line (HCC-T) from a patient with hepatoma bearing no evidence of hepatitis B or a virus infection', Cancer, vol. 64, no. 5, pp. 1054-1060.
Saito, Hidetsugu ; Morizane, T. ; Watanabe, T. ; Kagawa, T. ; Iwabuchi, N. ; Kumagai, N. ; Inagaki, Y. ; Tsuchimoto, K. ; Tsuchiya, M. / Establishment of a human cell line (HCC-T) from a patient with hepatoma bearing no evidence of hepatitis B or a virus infection. In: Cancer. 1989 ; Vol. 64, No. 5. pp. 1054-1060.
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AU - Kagawa, T.

AU - Iwabuchi, N.

AU - Kumagai, N.

AU - Inagaki, Y.

AU - Tsuchimoto, K.

AU - Tsuchiya, M.

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