Establishment of a human cell line (HCC-T) from a patient with hepatoma bearing no evidence of hepatitis B or a virus infection

Hidetsugu Saito, T. Morizane, T. Watanabe, T. Kagawa, N. Iwabuchi, N. Kumagai, Y. Inagaki, K. Tsuchimoto, M. Tsuchiya

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Abstract

A human hepatoma cell line, designated HCC-T, was established. The tumor was surgically obtained from a Japanese male patient with hepatocellular carcinoma (HCC) arising in a cirrhotic liver that had supposedly developed from nonAnonB (NANB) chronic hepatitis. HCC-T exhibited a typical morphology of epithelial cells in culture. Population doubling time was 24 hours and HCC-T cells had characteristics of malignant cells demonstrated by the ability to grow in a soft agar medium and transplantability to nude mice. The histologic condition of the tumor transplanted to a nude mouse showed similarity to the original tumor. A chromosome analysis showed that there were ten identifiable marker chromosomes and sex chromosomes with its modal number of 64. Alpha-fetoprotein (AFP) production was demonstrated by direct immunofluorescence study, but albumin or hepatitis B surface antigen were not detectable. The integration of hepatitis B viral DNA was not demonstrable in the genome of HCC-T cells or the original hepatoma.

Original languageEnglish
Pages (from-to)1054-1060
Number of pages7
JournalCancer
Volume64
Issue number5
Publication statusPublished - 1989

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ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Saito, H., Morizane, T., Watanabe, T., Kagawa, T., Iwabuchi, N., Kumagai, N., Inagaki, Y., Tsuchimoto, K., & Tsuchiya, M. (1989). Establishment of a human cell line (HCC-T) from a patient with hepatoma bearing no evidence of hepatitis B or a virus infection. Cancer, 64(5), 1054-1060.