Establishment of a human hepatoma cell line, HCC-T

Hidetsugu Saito, Toshio Morizane, Shingo Matsumoto, Naoto Iwabuchi, Itsurou Satoh, Masaya Oda, Masaharu Tsuchiya, Tetsu Watanabe, Toshiharu Tsuzuki

Research output: Contribution to journalArticlepeer-review

Abstract

A continuous human cell line designated HCC-T was established from the hepatocelluar carcinoma (HCC) of a 69-year-old Japanese male patient. Serum hepatitis A antibody, hepatitis B surface, e and core antigens and antibodies to those viral antigens were all negative in this patient. He had no histories of a large alcoholic consumption and blood transfusion. It is thought that the HCC had developed from liver cirrhosis which was a sequela of nonA nonB type chronic hepatitis. HCC-T cells were cultured in tissue culture flasks attaching to the bottom, in RPMI-1640 medium supplemented with 10% fetal calf serum. The population doubling time of HCC-T cell was estimated as 24h in this culture medium. Morphological study based on the light and electron microscopies revealed that HCC-T cells were HCC cells of epithelial-like shape. Intracellular production of alphafetoprotein was demonstrated by direct immumofluorescence technique. The chromosome analysis revealed that HCC-T had a male sex chromosome and a chromosome mode of 64. It was demonstrated that HCC-T cells were able to grow without anchoring dependence in a soft agar medium. When HCC-T cells were subcutaneously transplanted to athymic BALB/c nude mice, tumors developed at sites and minimum transplantable cell number was 2×106. Light microscopic observation showed that the transplanted tumor tissue was similar in morphology to the original HCC tissue.

Original languageEnglish
Pages (from-to)1664-1671
Number of pages8
JournalNippon Shokakibyo Gakkai Zasshi
Volume85
Issue number9
DOIs
Publication statusPublished - 1988 Jan 1

Keywords

  • hepatocellular carcinoma
  • human cell line
  • nonA nonB

ASJC Scopus subject areas

  • Gastroenterology

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