Establishment of a model of sentinel lymph node metastasis using immunodeficient swine

Toshiaki Kurihara, Sachiko Matsuda, Yuki Nakamura, Shunichi Suzuki, Daiichiro Fuchimoto, Akira Onishi, Kohei Saeki, Takayuki Nakagawa, Reina Fujiwara, Masatoshi Kamata, Junko Kuramoto, Kaori Kameyama, Masaki Sekino, Moriaki Kusakabe, Tetsu Hayashida, Hiromitsu Jinno, Yuukou Kitagawa

Research output: Contribution to journalArticle

Abstract

Lymph node metastasis occurs via the migration of cancer cells through the lymphatic system. Sentinel lymph node (SLN) biopsy is a common diagnostic strategy. SLNs have been studied using healthy rodents and large animals without metastasis. Here we used immunodeficient swine to establish a model of lymph node metastasis. We used RAG2-knockout immunodeficient swine. A431 human epithelial carcinoma cells expressing green fluorescent protein were injected subcutaneously into the posterior sides of the auricle, forelimb and hindlimb of knockout swine. Indigo carmine dye was injected subcutaneously 8 weeks after tumour cell transplantation. SLNs were extracted, observed using a stereoscopic fluorescence microscope and analysed histologically using haematoxylin and eosin staining, and immunohistochemistry. Lymphoid follicles were found in wild-type swine, and a few aggregated lymphocytes and immature lymphoid follicles were observed in knockout swine. Fluorescence in the lymph nodes indicated metastasis of tumour cells to the lymph nodes. Tumour cells replaced lymph node architectures, showed high-grade nuclear atypia and formed irregular tumour nests. Our model may be useful for the preclinical validation of diagnostic methods and minimally invasive treatment of metastatic cancer.

Original languageEnglish
Number of pages1
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - 2019 May 28

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Swine
Neoplasm Metastasis
Lymph Nodes
Neoplasms
Fluorescence
Indigo Carmine
Sentinel Lymph Node Biopsy
Lymphatic System
Forelimb
Cell Transplantation
Hematoxylin
Eosine Yellowish-(YS)
Hindlimb
Green Fluorescent Proteins
Cell Movement
Sentinel Lymph Node
Rodentia
Coloring Agents
Epithelial Cells
Immunohistochemistry

ASJC Scopus subject areas

  • General

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Establishment of a model of sentinel lymph node metastasis using immunodeficient swine. / Kurihara, Toshiaki; Matsuda, Sachiko; Nakamura, Yuki; Suzuki, Shunichi; Fuchimoto, Daiichiro; Onishi, Akira; Saeki, Kohei; Nakagawa, Takayuki; Fujiwara, Reina; Kamata, Masatoshi; Kuramoto, Junko; Kameyama, Kaori; Sekino, Masaki; Kusakabe, Moriaki; Hayashida, Tetsu; Jinno, Hiromitsu; Kitagawa, Yuukou.

In: Scientific reports, Vol. 9, No. 1, 28.05.2019.

Research output: Contribution to journalArticle

Kurihara, T, Matsuda, S, Nakamura, Y, Suzuki, S, Fuchimoto, D, Onishi, A, Saeki, K, Nakagawa, T, Fujiwara, R, Kamata, M, Kuramoto, J, Kameyama, K, Sekino, M, Kusakabe, M, Hayashida, T, Jinno, H & Kitagawa, Y 2019, 'Establishment of a model of sentinel lymph node metastasis using immunodeficient swine', Scientific reports, vol. 9, no. 1. https://doi.org/10.1038/s41598-019-44171-w
Kurihara, Toshiaki ; Matsuda, Sachiko ; Nakamura, Yuki ; Suzuki, Shunichi ; Fuchimoto, Daiichiro ; Onishi, Akira ; Saeki, Kohei ; Nakagawa, Takayuki ; Fujiwara, Reina ; Kamata, Masatoshi ; Kuramoto, Junko ; Kameyama, Kaori ; Sekino, Masaki ; Kusakabe, Moriaki ; Hayashida, Tetsu ; Jinno, Hiromitsu ; Kitagawa, Yuukou. / Establishment of a model of sentinel lymph node metastasis using immunodeficient swine. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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