Establishment of a new detection system for the dimerization of IRE1α by BiFC assay

Satoko Shinjo, Etsu Tashiro, Masaya Imoto

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


We developed a new detection system for the activation of an endoplasmic reticulum (ER) stress sensor, inositol requiring kinase 1 α (IRE1α), by evaluating dimerization of it by bimolecular fluorescence complementation (BiFC) assay. By detecting the fluorescence derived from the reconstituted cerulean, this assay system enabled us to distinguish the activation behaviors of IRE1α as to ER stress-inducing compounds.

Original languageEnglish
Pages (from-to)1333-1336
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Issue number6
Publication statusPublished - 2013


  • (IRE1α)
  • Bimolecular fluorescent complementation (BiFC) assay
  • Imaging
  • Inositol requiring kinase 1 α
  • Small molecule
  • Unfolded protein response (UPR)

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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