Establishment of a new human acute monocytic leukemia cell line TZ-1 with t(1;11)(p32;q23) and fusion gene MLL-EPS15

M. Sagawa, T. Shimizu, Takayuki Shimizu, N. Awaya, Takayuki Mitsuhashi, Y. Ikeda, Shinichiro Okamoto, M. Kizaki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Human leukemia cell lines are of great value in investigating basic and applied aspects of cell biology and clinical medicine. There have been 37 leukemia cell lines carrying 11q23 translocation and MLL rearrangements; however, cell lines harboring with t(1;11)(p32;q23) have not been established. We report here for the first time a new acute monocytic leukemia (AMoL) cell line with t(1;11)(p32;q23), designated TZ-1, and herein describe its biological characteristics. Mononuclear cells isolated from the ascites from a patient with AMoL (French-American-British classification; acute myeloid leukemia M5a) were isolated and passaged by liquid culture medium for a year. TZ-1 cells revealed typical monocytic features in morphology and had a t(1;11)(p32;q23) translocation. The immunoprofiling as determined by flow cytometry showed that TZ-1 cells are positive for myeloid and monocytic markers with lymphoid-associated markers. Fluorescence in situ hybridization and reverse transcription-polymerase chain reaction analyses revealed MLL-EPS15 fusion transcript and protein. Taken together, these results suggest that TZ-1 is a new monocytic leukemia cell line with t(1;11) translocation and fusion gene MLL-EPS15. The established cell line, TZ-1, could provide a valuable model in the analysis of the pathogenesis of MLL-EPS15-positive leukemia and in the development of new agents for this type of leukemia.

Original languageEnglish
Pages (from-to)1566-1571
Number of pages6
JournalLeukemia
Volume20
Issue number9
DOIs
Publication statusPublished - 2006 Sep

Fingerprint

Leukemia, Monocytic, Acute
Gene Fusion
Leukemia
Cell Line
Clinical Medicine
Myeloid Cells
Fluorescence In Situ Hybridization
Ascites
Acute Myeloid Leukemia
Reverse Transcription
Cell Biology
Culture Media
Flow Cytometry
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

Cite this

Establishment of a new human acute monocytic leukemia cell line TZ-1 with t(1;11)(p32;q23) and fusion gene MLL-EPS15. / Sagawa, M.; Shimizu, T.; Shimizu, Takayuki; Awaya, N.; Mitsuhashi, Takayuki; Ikeda, Y.; Okamoto, Shinichiro; Kizaki, M.

In: Leukemia, Vol. 20, No. 9, 09.2006, p. 1566-1571.

Research output: Contribution to journalArticle

@article{b30385c31d2d4b55a6437e6434b4991d,
title = "Establishment of a new human acute monocytic leukemia cell line TZ-1 with t(1;11)(p32;q23) and fusion gene MLL-EPS15",
abstract = "Human leukemia cell lines are of great value in investigating basic and applied aspects of cell biology and clinical medicine. There have been 37 leukemia cell lines carrying 11q23 translocation and MLL rearrangements; however, cell lines harboring with t(1;11)(p32;q23) have not been established. We report here for the first time a new acute monocytic leukemia (AMoL) cell line with t(1;11)(p32;q23), designated TZ-1, and herein describe its biological characteristics. Mononuclear cells isolated from the ascites from a patient with AMoL (French-American-British classification; acute myeloid leukemia M5a) were isolated and passaged by liquid culture medium for a year. TZ-1 cells revealed typical monocytic features in morphology and had a t(1;11)(p32;q23) translocation. The immunoprofiling as determined by flow cytometry showed that TZ-1 cells are positive for myeloid and monocytic markers with lymphoid-associated markers. Fluorescence in situ hybridization and reverse transcription-polymerase chain reaction analyses revealed MLL-EPS15 fusion transcript and protein. Taken together, these results suggest that TZ-1 is a new monocytic leukemia cell line with t(1;11) translocation and fusion gene MLL-EPS15. The established cell line, TZ-1, could provide a valuable model in the analysis of the pathogenesis of MLL-EPS15-positive leukemia and in the development of new agents for this type of leukemia.",
author = "M. Sagawa and T. Shimizu and Takayuki Shimizu and N. Awaya and Takayuki Mitsuhashi and Y. Ikeda and Shinichiro Okamoto and M. Kizaki",
year = "2006",
month = "9",
doi = "10.1038/sj.leu.2404305",
language = "English",
volume = "20",
pages = "1566--1571",
journal = "Leukemia",
issn = "0887-6924",
publisher = "Nature Publishing Group",
number = "9",

}

TY - JOUR

T1 - Establishment of a new human acute monocytic leukemia cell line TZ-1 with t(1;11)(p32;q23) and fusion gene MLL-EPS15

AU - Sagawa, M.

AU - Shimizu, T.

AU - Shimizu, Takayuki

AU - Awaya, N.

AU - Mitsuhashi, Takayuki

AU - Ikeda, Y.

AU - Okamoto, Shinichiro

AU - Kizaki, M.

PY - 2006/9

Y1 - 2006/9

N2 - Human leukemia cell lines are of great value in investigating basic and applied aspects of cell biology and clinical medicine. There have been 37 leukemia cell lines carrying 11q23 translocation and MLL rearrangements; however, cell lines harboring with t(1;11)(p32;q23) have not been established. We report here for the first time a new acute monocytic leukemia (AMoL) cell line with t(1;11)(p32;q23), designated TZ-1, and herein describe its biological characteristics. Mononuclear cells isolated from the ascites from a patient with AMoL (French-American-British classification; acute myeloid leukemia M5a) were isolated and passaged by liquid culture medium for a year. TZ-1 cells revealed typical monocytic features in morphology and had a t(1;11)(p32;q23) translocation. The immunoprofiling as determined by flow cytometry showed that TZ-1 cells are positive for myeloid and monocytic markers with lymphoid-associated markers. Fluorescence in situ hybridization and reverse transcription-polymerase chain reaction analyses revealed MLL-EPS15 fusion transcript and protein. Taken together, these results suggest that TZ-1 is a new monocytic leukemia cell line with t(1;11) translocation and fusion gene MLL-EPS15. The established cell line, TZ-1, could provide a valuable model in the analysis of the pathogenesis of MLL-EPS15-positive leukemia and in the development of new agents for this type of leukemia.

AB - Human leukemia cell lines are of great value in investigating basic and applied aspects of cell biology and clinical medicine. There have been 37 leukemia cell lines carrying 11q23 translocation and MLL rearrangements; however, cell lines harboring with t(1;11)(p32;q23) have not been established. We report here for the first time a new acute monocytic leukemia (AMoL) cell line with t(1;11)(p32;q23), designated TZ-1, and herein describe its biological characteristics. Mononuclear cells isolated from the ascites from a patient with AMoL (French-American-British classification; acute myeloid leukemia M5a) were isolated and passaged by liquid culture medium for a year. TZ-1 cells revealed typical monocytic features in morphology and had a t(1;11)(p32;q23) translocation. The immunoprofiling as determined by flow cytometry showed that TZ-1 cells are positive for myeloid and monocytic markers with lymphoid-associated markers. Fluorescence in situ hybridization and reverse transcription-polymerase chain reaction analyses revealed MLL-EPS15 fusion transcript and protein. Taken together, these results suggest that TZ-1 is a new monocytic leukemia cell line with t(1;11) translocation and fusion gene MLL-EPS15. The established cell line, TZ-1, could provide a valuable model in the analysis of the pathogenesis of MLL-EPS15-positive leukemia and in the development of new agents for this type of leukemia.

UR - http://www.scopus.com/inward/record.url?scp=33747619161&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747619161&partnerID=8YFLogxK

U2 - 10.1038/sj.leu.2404305

DO - 10.1038/sj.leu.2404305

M3 - Article

C2 - 16826222

AN - SCOPUS:33747619161

VL - 20

SP - 1566

EP - 1571

JO - Leukemia

JF - Leukemia

SN - 0887-6924

IS - 9

ER -