Establishment of a novel childhood acute myeloid leukaemia cell line, KOPM-88, containing partial tandem duplication of the MLL gene and an in vivo model for childhood acute myeloid leukaemia using NOD/SCID mice

Mutsumi Hayashi, Kensuke Kondoh, Yuji Nakata, Akitoshi Kinoshita, Taijiro Mori, Takao Takahashi, Michiie Sakamoto, Taketo Yamada

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

MLL gene rearrangement is common in both adult and childhood acute myeloid leukaemia (AML), and its role in oncogenesis has been investigated. While over 50 translocated-partner genes have been identified so far, few studies have detailed the molecular mechanism of partial tandem duplication (PTD) of the MLL gene. The prognostic impact and contribution to leukaemogenesis of MLL-PTD, especially in childhood cases, remain unknown. We have established a novel cell line containing MLL-PTD derived from an 11-year-old patient with AML and designated as KOPM-88. KOPM-88 cells exhibited certain characteristics associated with the myeloid lineage including abundant primary granules in the cytoplasm and the expression of myeloperoxidase. The cell growth of KOPM-88 was cytokine independent but was accelerated by granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor. MLL-PTD of exon 2 to exon 6 and exon 2 to exon 8 was revealed using Southern blotting, fluorescence in situ hybridisation, and reverse transcription polymerase chain reaction/DNA sequencing. Furthermore, non-obese diabetic/severe combined immunodeficient mice inoculated with KOPM-88 cells exhibited leukaemic infiltrations in the bone marrow and hemiparalysis because of compression myelopathy. This is the first report of an in vivo animal model exhibiting the systemic involvement of childhood AML containing MLL-PTD. KOPM-88 cells and our murine model may be useful for investigating the pathogenesis of childhood AML associated with MLL gene rearrangement.

Original languageEnglish
Pages (from-to)221-232
Number of pages12
JournalBritish Journal of Haematology
Volume137
Issue number3
DOIs
Publication statusPublished - 2007 May

Fingerprint

Inbred NOD Mouse
Gene Duplication
SCID Mice
Myeloid Cells
Acute Myeloid Leukemia
Exons
Cell Line
Gene Rearrangement
Hemiplegia
Spinal Cord Diseases
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Southern Blotting
Fluorescence In Situ Hybridization
DNA Sequence Analysis
Peroxidase
Reverse Transcription
Carcinogenesis
Cytoplasm
Animal Models

Keywords

  • Animal model
  • Cell line
  • Childhood acute myeloid leukaemia
  • MLL gene
  • Partial tandem duplication

ASJC Scopus subject areas

  • Hematology

Cite this

Establishment of a novel childhood acute myeloid leukaemia cell line, KOPM-88, containing partial tandem duplication of the MLL gene and an in vivo model for childhood acute myeloid leukaemia using NOD/SCID mice. / Hayashi, Mutsumi; Kondoh, Kensuke; Nakata, Yuji; Kinoshita, Akitoshi; Mori, Taijiro; Takahashi, Takao; Sakamoto, Michiie; Yamada, Taketo.

In: British Journal of Haematology, Vol. 137, No. 3, 05.2007, p. 221-232.

Research output: Contribution to journalArticle

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abstract = "MLL gene rearrangement is common in both adult and childhood acute myeloid leukaemia (AML), and its role in oncogenesis has been investigated. While over 50 translocated-partner genes have been identified so far, few studies have detailed the molecular mechanism of partial tandem duplication (PTD) of the MLL gene. The prognostic impact and contribution to leukaemogenesis of MLL-PTD, especially in childhood cases, remain unknown. We have established a novel cell line containing MLL-PTD derived from an 11-year-old patient with AML and designated as KOPM-88. KOPM-88 cells exhibited certain characteristics associated with the myeloid lineage including abundant primary granules in the cytoplasm and the expression of myeloperoxidase. The cell growth of KOPM-88 was cytokine independent but was accelerated by granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor. MLL-PTD of exon 2 to exon 6 and exon 2 to exon 8 was revealed using Southern blotting, fluorescence in situ hybridisation, and reverse transcription polymerase chain reaction/DNA sequencing. Furthermore, non-obese diabetic/severe combined immunodeficient mice inoculated with KOPM-88 cells exhibited leukaemic infiltrations in the bone marrow and hemiparalysis because of compression myelopathy. This is the first report of an in vivo animal model exhibiting the systemic involvement of childhood AML containing MLL-PTD. KOPM-88 cells and our murine model may be useful for investigating the pathogenesis of childhood AML associated with MLL gene rearrangement.",
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AU - Kondoh, Kensuke

AU - Nakata, Yuji

AU - Kinoshita, Akitoshi

AU - Mori, Taijiro

AU - Takahashi, Takao

AU - Sakamoto, Michiie

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