An axenic culture of Entamoeba histolytica was obtained from cysts in stool bypassing bacteria-associated cultivation of trophozoites. The cysts were isolated from the stool of an asymptomatic carrier by a series of density gradient centrifugations. After incubation in 1% HCl, they were washed, transferred into BI-S-33 medium containing epimastigotes of Trypanosoma cruzi, and placed at 35.5 C. In a few days, actively motile trophozoites of amoeba could be observed. After 37 transfers, amoebae in one of the two monoxenic cultures, which could grow as rapidly as axenic E. histolytica, were inoculated into BI-S-33 medium with no trypanosomatid associates. Subsequent observations for longer than 9 months indicated that the amoebae grew under the axenic condition. The amoebae were identified as E. histolytica by the gel diffusion precipitin test.
|Number of pages||6|
|Journal||Japanese Journal of Parasitology|
|Publication status||Published - 1983 Jan 1|
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