Establishment of an axenic strain of Entamoeba histolytica from cysts in stool, bypassing bacteria-associated cultivation

S. Kobayashi, T. Takeuchi

Research output: Contribution to journalArticle

Abstract

An axenic culture of Entamoeba histolytica was obtained from cysts in stool bypassing bacteria-associated cultivation of trophozoites. The cysts were isolated from the stool of an asymptomatic carrier by a series of density gradient centrifugations. After incubation in 1% HCl, they were washed, transferred into BI-S-33 medium containing epimastigotes of Trypanosoma cruzi, and placed at 35.5 C. In a few days, actively motile trophozoites of amoeba could be observed. After 37 transfers, amoebae in one of the two monoxenic cultures, which could grow as rapidly as axenic E. histolytica, were inoculated into BI-S-33 medium with no trypanosomatid associates. Subsequent observations for longer than 9 months indicated that the amoebae grew under the axenic condition. The amoebae were identified as E. histolytica by the gel diffusion precipitin test.

Original languageEnglish
Pages (from-to)475-480
Number of pages6
JournalJapanese Journal of Parasitology
Volume32
Issue number5
Publication statusPublished - 1983 Jan 1

ASJC Scopus subject areas

  • Parasitology

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