Establishment of an ovarian metastasis model and possible involvement of E-cadherin down-regulation in the metastasis

Yoshiko Kuwabara, Taketo Yamada, Ken Yamazaki, Wen Lin Du, Kouji Banno, Daisuke Aoki, Michiie Sakamoto

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22 Citations (Scopus)

Abstract

Clinical observations of cases of ovarian metastasis suggest that there may be a unique mechanism underlying ovarian-specific metastasis. This study was undertaken to establish an in vivo model of metastasis to the ovary, and to investigate the mechanism of ovarian-specific metastasis. We examined the capacity for ovarian metastasis in eight different human carcinoma cell lines by implantation in female NOD/SCID mice transvenously and intraperitoneally. By transvenous inoculation, only RERF-LC-AI, a poorly differentiated carcinoma cell line, frequently demonstrated ovarian metastasis. By intraperitoneal inoculation, four of the eight cell lines (HGC27, MKN-45, KATO-III, and RERF-LC-AI) metastasized to the ovary. We compared E-cadherin expression among ovarian metastatic cell lines and others. All of these four ovarian metastatic cell lines and HSKTC, a Krukenberg tumor cell line, showed E-cadherin down-regulation and others did not. E-cadherin was then forcibly expressed in RERF-LC-AI, and inhibited ovarian metastasis completely. The capacity for metastasizing to the other organs was not affected by E-cadherin expression. We also performed histological investigation of clinical ovarian-metastatic tumor cases. About half of all ovarian-metastatic tumor cases showed loss or reduction of E-cadherin expression. These data suggest that E-cadherin down-regulation may be involved in ovarian-specific metastasis.

Original languageEnglish
Pages (from-to)1933-1939
Number of pages7
JournalCancer science
Volume99
Issue number10
DOIs
Publication statusPublished - 2008 Nov 3

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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