Abstract
Tumor cell variants were established in vitro by treating a metastatic variant of murine colon adenocarcinoma 26 (P-26-select) in serum-free RPMI 1640 medium for 6 to 10 weeks. Five tumor cell lines were established from independent cultures and designated PS-1 to PS-5. The PS cells showed higher growth potential in vitro under serum-free or low-serum conditions than the parental P-26-select. In comparison to P-26-select, the PS cell lines possessed enhanced lung-colonizing ability after i.v. inoculation and metastasized spontaneously to the lung following s.c. inoculation into the flank or the right fore-footpads of BALB/c mice. Metastatic nodules were also observed in the liver. Spleens and livers of the tumor-bearing mice were enlarged mainly as a result of hematopoiesis, suggesting the production of CSF-like substance(s) by the tumor cells. The PS cells secreted increased amounts of putative autocrine growth factor(s) and CSF-like substance(s) in vitro. These characteristics might be related to the in vivo metastatic ability of the PS cells.
| Original language | English |
|---|---|
| Pages (from-to) | 118-124 |
| Number of pages | 7 |
| Journal | International Journal of Cancer |
| Volume | 46 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1990 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Establishment of highly metastatic variants of murine colon adenocarcinoma 26 under serum-free conditions. / Sugimoto, Yoshikazu; Oh-hara, T.; Heike, Y.; Watanabe, M.; Nakatsuru, Y.; Ishikawa, T.; Tsuruo, T.
In: International Journal of Cancer, Vol. 46, No. 1, 1990, p. 118-124.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Establishment of highly metastatic variants of murine colon adenocarcinoma 26 under serum-free conditions
AU - Sugimoto, Yoshikazu
AU - Oh-hara, T.
AU - Heike, Y.
AU - Watanabe, M.
AU - Nakatsuru, Y.
AU - Ishikawa, T.
AU - Tsuruo, T.
PY - 1990
Y1 - 1990
N2 - Tumor cell variants were established in vitro by treating a metastatic variant of murine colon adenocarcinoma 26 (P-26-select) in serum-free RPMI 1640 medium for 6 to 10 weeks. Five tumor cell lines were established from independent cultures and designated PS-1 to PS-5. The PS cells showed higher growth potential in vitro under serum-free or low-serum conditions than the parental P-26-select. In comparison to P-26-select, the PS cell lines possessed enhanced lung-colonizing ability after i.v. inoculation and metastasized spontaneously to the lung following s.c. inoculation into the flank or the right fore-footpads of BALB/c mice. Metastatic nodules were also observed in the liver. Spleens and livers of the tumor-bearing mice were enlarged mainly as a result of hematopoiesis, suggesting the production of CSF-like substance(s) by the tumor cells. The PS cells secreted increased amounts of putative autocrine growth factor(s) and CSF-like substance(s) in vitro. These characteristics might be related to the in vivo metastatic ability of the PS cells.
AB - Tumor cell variants were established in vitro by treating a metastatic variant of murine colon adenocarcinoma 26 (P-26-select) in serum-free RPMI 1640 medium for 6 to 10 weeks. Five tumor cell lines were established from independent cultures and designated PS-1 to PS-5. The PS cells showed higher growth potential in vitro under serum-free or low-serum conditions than the parental P-26-select. In comparison to P-26-select, the PS cell lines possessed enhanced lung-colonizing ability after i.v. inoculation and metastasized spontaneously to the lung following s.c. inoculation into the flank or the right fore-footpads of BALB/c mice. Metastatic nodules were also observed in the liver. Spleens and livers of the tumor-bearing mice were enlarged mainly as a result of hematopoiesis, suggesting the production of CSF-like substance(s) by the tumor cells. The PS cells secreted increased amounts of putative autocrine growth factor(s) and CSF-like substance(s) in vitro. These characteristics might be related to the in vivo metastatic ability of the PS cells.
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U2 - 10.1002/ijc.2910460122
DO - 10.1002/ijc.2910460122
M3 - Article
VL - 46
SP - 118
EP - 124
JO - International Journal of Cancer
JF - International Journal of Cancer
SN - 0020-7136
IS - 1
ER -