Establishment of highly metastatic variants of murine colon adenocarcinoma 26 under serum-free conditions

Yoshikazu Sugimoto, T. Oh-hara, Y. Heike, M. Watanabe, Y. Nakatsuru, T. Ishikawa, T. Tsuruo

Research output: Contribution to journalArticle

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Abstract

Tumor cell variants were established in vitro by treating a metastatic variant of murine colon adenocarcinoma 26 (P-26-select) in serum-free RPMI 1640 medium for 6 to 10 weeks. Five tumor cell lines were established from independent cultures and designated PS-1 to PS-5. The PS cells showed higher growth potential in vitro under serum-free or low-serum conditions than the parental P-26-select. In comparison to P-26-select, the PS cell lines possessed enhanced lung-colonizing ability after i.v. inoculation and metastasized spontaneously to the lung following s.c. inoculation into the flank or the right fore-footpads of BALB/c mice. Metastatic nodules were also observed in the liver. Spleens and livers of the tumor-bearing mice were enlarged mainly as a result of hematopoiesis, suggesting the production of CSF-like substance(s) by the tumor cells. The PS cells secreted increased amounts of putative autocrine growth factor(s) and CSF-like substance(s) in vitro. These characteristics might be related to the in vivo metastatic ability of the PS cells.

Original languageEnglish
Pages (from-to)118-124
Number of pages7
JournalInternational Journal of Cancer
Volume46
Issue number1
DOIs
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

Colon
Adenocarcinoma
Serum
Neoplasms
Lung
Liver
Hematopoiesis
Tumor Cell Line
Intercellular Signaling Peptides and Proteins
Spleen
Cell Line
Growth
aliflurane
In Vitro Techniques

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Establishment of highly metastatic variants of murine colon adenocarcinoma 26 under serum-free conditions. / Sugimoto, Yoshikazu; Oh-hara, T.; Heike, Y.; Watanabe, M.; Nakatsuru, Y.; Ishikawa, T.; Tsuruo, T.

In: International Journal of Cancer, Vol. 46, No. 1, 1990, p. 118-124.

Research output: Contribution to journalArticle

Sugimoto, Yoshikazu ; Oh-hara, T. ; Heike, Y. ; Watanabe, M. ; Nakatsuru, Y. ; Ishikawa, T. ; Tsuruo, T. / Establishment of highly metastatic variants of murine colon adenocarcinoma 26 under serum-free conditions. In: International Journal of Cancer. 1990 ; Vol. 46, No. 1. pp. 118-124.
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