Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins

Hayato Fukusumi, Tomoko Shofuda, Yohei Bamba, Atsuyo Yamamoto, Daisuke Kanematsu, Yukako Handa, Keisuke Okita, Masaya Nakamura, Shinya Yamanaka, Hideyuki Okano, Yonehiro Kanemura

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Human neural progenitor cells (hNPCs) have previously been generated from limited numbers of human induced pluripotent stem cell (hiPSC) clones. Here, 21 hiPSC clones derived from human dermal fibroblasts, cord blood cells, and peripheral blood mononuclear cells were differentiated using two neural induction methods, an embryoid body (EB) formation-based method and an EB formation method using dual SMAD inhibitors (dSMADi). Our results showed that expandable hNPCs could be generated from hiPSC clones with diverse somatic tissue origins. The established hNPCs exhibited a mid/hindbrain-type neural identity and uniform expression of neural progenitor genes.

Original languageEnglish
Article number7235757
JournalStem Cells International
Volume2016
DOIs
Publication statusPublished - 2016

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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    Fukusumi, H., Shofuda, T., Bamba, Y., Yamamoto, A., Kanematsu, D., Handa, Y., Okita, K., Nakamura, M., Yamanaka, S., Okano, H., & Kanemura, Y. (2016). Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins. Stem Cells International, 2016, [7235757]. https://doi.org/10.1155/2016/7235757