Establishment of In Vitro FUS-Associated Familial Amyotrophic Lateral Sclerosis Model Using Human Induced Pluripotent Stem Cells

Naoki Ichiyanagi, Koki Fujimori, Masato Yano, Chikako Ishihara-Fujisaki, Takefumi Sone, Tetsuya Akiyama, Yohei Okada, Wado Akamatsu, Takuya Matsumoto, Mitsuru Ishikawa, Yoshinori Nishimoto, Yasuharu Ishihara, Tetsushi Sakuma, Takashi Yamamoto, Hitomi Tsuiji, Naoki Suzuki, Hitoshi Warita, Masashi Aoki, Hideyuki Okano

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Amyotrophic lateral sclerosis (ALS) is a late-onset motor neuron disorder. Although its neuropathology is well understood, the cellular and molecular mechanisms are yet to be elucidated due to limitations in the currently available human genetic data. In this study, we generated induced pluripotent stem cells (iPSC) from two familial ALS (FALS) patients with a missense mutation in the fused-in sarcoma (FUS) gene carrying the heterozygous FUS H517D mutation, and isogenic iPSCs with the homozygous FUS H517D mutation by genome editing technology. These cell-derived motor neurons mimicked several neurodegenerative phenotypes including mis-localization of FUS into cytosolic and stress granules under stress conditions, and cellular vulnerability. Moreover, exon array analysis using motor neuron precursor cells (MPCs) combined with CLIP-seq datasets revealed aberrant gene expression and/or splicing pattern in FALS MPCs. These results suggest that iPSC-derived motor neurons are a useful tool for analyzing the pathogenesis of human motor neuron disorders. Okano, Yano, and colleagues established iPSCs from two familial ALS patients with the missense mutation in the FUS gene and differentiated them into motor neurons as a novel in vitro model for ALS. Moreover, this model enables the pursuit of correlations and new discovery of molecular pathophysiology in various cell biological phenomena by endogenous mutation in patient-derived motor neurons.

Original languageEnglish
JournalStem Cell Reports
DOIs
Publication statusAccepted/In press - 2015 May 20

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Induced Pluripotent Stem Cells
Motor Neurons
Stem cells
Sarcoma
Neurons
Genes
Amyotrophic Lateral Sclerosis
Missense Mutation
Mutation
Biological Phenomena
Amyotrophic lateral sclerosis 1
In Vitro Techniques
Medical Genetics
Gene expression
Exons
Technology
Phenotype
Gene Expression

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Developmental Biology
  • Genetics

Cite this

Establishment of In Vitro FUS-Associated Familial Amyotrophic Lateral Sclerosis Model Using Human Induced Pluripotent Stem Cells. / Ichiyanagi, Naoki; Fujimori, Koki; Yano, Masato; Ishihara-Fujisaki, Chikako; Sone, Takefumi; Akiyama, Tetsuya; Okada, Yohei; Akamatsu, Wado; Matsumoto, Takuya; Ishikawa, Mitsuru; Nishimoto, Yoshinori; Ishihara, Yasuharu; Sakuma, Tetsushi; Yamamoto, Takashi; Tsuiji, Hitomi; Suzuki, Naoki; Warita, Hitoshi; Aoki, Masashi; Okano, Hideyuki.

In: Stem Cell Reports, 20.05.2015.

Research output: Contribution to journalArticle

Ichiyanagi, N, Fujimori, K, Yano, M, Ishihara-Fujisaki, C, Sone, T, Akiyama, T, Okada, Y, Akamatsu, W, Matsumoto, T, Ishikawa, M, Nishimoto, Y, Ishihara, Y, Sakuma, T, Yamamoto, T, Tsuiji, H, Suzuki, N, Warita, H, Aoki, M & Okano, H 2015, 'Establishment of In Vitro FUS-Associated Familial Amyotrophic Lateral Sclerosis Model Using Human Induced Pluripotent Stem Cells', Stem Cell Reports. https://doi.org/10.1016/j.stemcr.2016.02.011
Ichiyanagi, Naoki ; Fujimori, Koki ; Yano, Masato ; Ishihara-Fujisaki, Chikako ; Sone, Takefumi ; Akiyama, Tetsuya ; Okada, Yohei ; Akamatsu, Wado ; Matsumoto, Takuya ; Ishikawa, Mitsuru ; Nishimoto, Yoshinori ; Ishihara, Yasuharu ; Sakuma, Tetsushi ; Yamamoto, Takashi ; Tsuiji, Hitomi ; Suzuki, Naoki ; Warita, Hitoshi ; Aoki, Masashi ; Okano, Hideyuki. / Establishment of In Vitro FUS-Associated Familial Amyotrophic Lateral Sclerosis Model Using Human Induced Pluripotent Stem Cells. In: Stem Cell Reports. 2015.
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