Establishment of live-cell-based coupled assay system for identification of compounds to modulate metabolic activities of cells

Kouichi Yanagi, Toru Komatsu, Shusuke Ogihara, Takayoshi Okabe, Hirotatsu Kojima, Tetsuo Nagano, Tasuku Ueno, Kenjiro Hanaoka, Yasuteru Urano

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, we present a live-cell-based fluorometric coupled assay system to identify the compounds that can regulate the targeted metabolic pathways in live cells. The assay is established through targeting specific metabolic pathways and using “input” and “output” metabolite pairs. The changes in the extracellular output that are generated and released into the extracellular media from the input are assessed as the activity of the pathway. The screening for the glycolytic pathway and amino acid metabolism reveals the activities of the present drugs, 6-BIO and regorafenib, that regulate the metabolic fate of tumor cells.

Original languageEnglish
Article number109311
JournalCell Reports
Volume36
Issue number1
DOIs
Publication statusPublished - 2021 Jul 6
Externally publishedYes

Keywords

  • chemical biology
  • coupled assays
  • fluorescent probes
  • glucolysis
  • glutaminolysis
  • metabolism

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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