Establishment of novel embryonic stem cell lines derived from the common marmoset (Callithrix jacchus)

Erika Sasaki, Kisaburo Hanazawa, Ryo Kurita, Akira Akatsuka, Takahito Yoshizaki, Hajime Ishii, Yoshikuni Tanioka, Yasuyuki Ohnishi, Hiroshi Suemizu, Ayako Sugawara, Norikazu Tamaoki, Kiyoko Izawa, Yukoh Nakazaki, Hiromi Hamada, Hirofumi Suemori, Shigetaka Asano, Norio Nakatsuji, Hideyuki Okano, Kenzaburo Tani

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

The successful establishment of human embryonic stem cell (hESC) lines has inaugurated a new era in regenerative medicine by facilitating the transplantation of differentiated ESCs to specific organs. However, problems with the safety and efficacy of hESC therapy in vivo remain to be resolved. Preclinical studies using animal model systems, including nonhuman primates, are essential to evaluate the safety and efficacy of hESC therapies. Previously, we demonstrated that common marmosets are suitable laboratory animal models for preclinical studies of hematopoietic stem cell therapies. As this animal model is also applicable to preclinical trials of ESC therapies, we have established novel common marmoset ESC (CMESC) lines. To obtain marmoset embryos, we developed a new embryo collection system, in which blastocysts can be obtained every 3 weeks from each marmoset pair. The inner cell mass was isolated by immunosurgery and plated on a mouse embryonic feeder layer. Some of the CMESC lines were cultured continuously for more than 1 year. These CMESC lines showed alkaline phosphatase activity and expressed stage-specific embryonic antigen (SSEA)-S, SSEA-4, TRA-1-60, and TRA-1-81. On the other hand, SSEA-1 was notdetected. Furthermore, our novel CMESCs are pluripotent, as evidenced by in vivo teratoma formation in immunodeficient mice and in vitro differentiation experiments. Our established CMESC lines and the common marmoset provide an excellent experimental model system for understanding differentiation mechanisms, as well as the development of regenerative therapies using hESCs.

Original languageEnglish
Pages (from-to)1304-1313
Number of pages10
JournalStem Cells
Volume23
Issue number9
DOIs
Publication statusPublished - 2005 Oct

Fingerprint

Callithrix
Embryonic Stem Cells
Cell Line
Cell- and Tissue-Based Therapy
Animal Models
Stage-Specific Embryonic Antigens
Embryonic Structures
CD15 Antigens
Feeder Cells
Safety
Regenerative Medicine
Teratoma
Blastocyst
Hematopoietic Stem Cells
Primates
Alkaline Phosphatase
Theoretical Models
Transplantation
Human Embryonic Stem Cells
Therapeutics

Keywords

  • Common marmoset
  • Embryoid body
  • Embryonic stem cells
  • Nonhuman primate
  • Teratoma formation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Establishment of novel embryonic stem cell lines derived from the common marmoset (Callithrix jacchus). / Sasaki, Erika; Hanazawa, Kisaburo; Kurita, Ryo; Akatsuka, Akira; Yoshizaki, Takahito; Ishii, Hajime; Tanioka, Yoshikuni; Ohnishi, Yasuyuki; Suemizu, Hiroshi; Sugawara, Ayako; Tamaoki, Norikazu; Izawa, Kiyoko; Nakazaki, Yukoh; Hamada, Hiromi; Suemori, Hirofumi; Asano, Shigetaka; Nakatsuji, Norio; Okano, Hideyuki; Tani, Kenzaburo.

In: Stem Cells, Vol. 23, No. 9, 10.2005, p. 1304-1313.

Research output: Contribution to journalArticle

Sasaki, E, Hanazawa, K, Kurita, R, Akatsuka, A, Yoshizaki, T, Ishii, H, Tanioka, Y, Ohnishi, Y, Suemizu, H, Sugawara, A, Tamaoki, N, Izawa, K, Nakazaki, Y, Hamada, H, Suemori, H, Asano, S, Nakatsuji, N, Okano, H & Tani, K 2005, 'Establishment of novel embryonic stem cell lines derived from the common marmoset (Callithrix jacchus)', Stem Cells, vol. 23, no. 9, pp. 1304-1313. https://doi.org/10.1634/stemcells.2004-0366
Sasaki, Erika ; Hanazawa, Kisaburo ; Kurita, Ryo ; Akatsuka, Akira ; Yoshizaki, Takahito ; Ishii, Hajime ; Tanioka, Yoshikuni ; Ohnishi, Yasuyuki ; Suemizu, Hiroshi ; Sugawara, Ayako ; Tamaoki, Norikazu ; Izawa, Kiyoko ; Nakazaki, Yukoh ; Hamada, Hiromi ; Suemori, Hirofumi ; Asano, Shigetaka ; Nakatsuji, Norio ; Okano, Hideyuki ; Tani, Kenzaburo. / Establishment of novel embryonic stem cell lines derived from the common marmoset (Callithrix jacchus). In: Stem Cells. 2005 ; Vol. 23, No. 9. pp. 1304-1313.
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