Estimating endogenous dopamine levels at D 2 and D 3 receptors in humans using the agonist radiotracer 11 C-(+)-PHNO

Fernando Caravaggio, Shinichiro Nakajima, Carol Borlido, Gary Remington, Philip Gerretsen, Alan Wilson, Sylvain Houle, Mahesh Menon, David Mamo, Ariel Graff-Guerrero

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Using positron emission tomography (PET) and an acute dopamine depletion challenge it is possible to estimate endogenous dopamine levels occupying dopamine D 2/3 receptors (D 2/3 R) in humans in vivo. Our group has developed 11 C-(+)-PHNO, the first agonist radiotracer with preferential in vivo affinity for D 3 R. Thus, the use of 11 C-(+)-PHNO offers the novel possibility of (i) estimating in vivo endogenous dopamine levels at D 2/3 R using an agonist radiotracer, and (ii) estimating endogenous dopamine levels at D 3 R in extrastriatal regions such as the substantia nigra, hypothalamus, and ventral pallidum. Ten healthy participants underwent a 11 C-(+)-PHNO PET scan under baseline conditions and another under acute endogenous dopamine depletion achieved via oral administration of alpha-methyl-para-tyrosine (64 mg/kg). 11 C-(+)-PHNO binding was sensitive to acute dopamine depletion, allowing in vivo estimates of endogenous dopamine in D 2 R-rich regions (caudate and putamen), mixed D 2/3 R-rich regions (ventral striatum and globus pallidus), and extrastriatal D 3 R-rich regions (hypothalamus and ventral pallidum). Dopamine depletion decreased self-reported vigor, which was correlated with the reduction in dopamine levels in the globus pallidus. 11 C-(+)-PHNO is a suitable radiotracer for use in estimating endogenous dopamine levels at D 2 R and D 3 R in neuropsychiatric populations.

Original languageEnglish
Pages (from-to)2769-2776
Number of pages8
JournalNeuropsychopharmacology
Volume39
Issue number12
DOIs
Publication statusPublished - 2014 Jan 1
Externally publishedYes

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Dopamine
Globus Pallidus
Positron-Emission Tomography
Hypothalamus
alpha-Methyltyrosine
Putamen
Substantia Nigra
Oral Administration
Healthy Volunteers

ASJC Scopus subject areas

  • Pharmacology
  • Psychiatry and Mental health

Cite this

Estimating endogenous dopamine levels at D 2 and D 3 receptors in humans using the agonist radiotracer 11 C-(+)-PHNO. / Caravaggio, Fernando; Nakajima, Shinichiro; Borlido, Carol; Remington, Gary; Gerretsen, Philip; Wilson, Alan; Houle, Sylvain; Menon, Mahesh; Mamo, David; Graff-Guerrero, Ariel.

In: Neuropsychopharmacology, Vol. 39, No. 12, 01.01.2014, p. 2769-2776.

Research output: Contribution to journalArticle

Caravaggio, F, Nakajima, S, Borlido, C, Remington, G, Gerretsen, P, Wilson, A, Houle, S, Menon, M, Mamo, D & Graff-Guerrero, A 2014, 'Estimating endogenous dopamine levels at D 2 and D 3 receptors in humans using the agonist radiotracer 11 C-(+)-PHNO', Neuropsychopharmacology, vol. 39, no. 12, pp. 2769-2776. https://doi.org/10.1038/npp.2014.125
Caravaggio, Fernando ; Nakajima, Shinichiro ; Borlido, Carol ; Remington, Gary ; Gerretsen, Philip ; Wilson, Alan ; Houle, Sylvain ; Menon, Mahesh ; Mamo, David ; Graff-Guerrero, Ariel. / Estimating endogenous dopamine levels at D 2 and D 3 receptors in humans using the agonist radiotracer 11 C-(+)-PHNO. In: Neuropsychopharmacology. 2014 ; Vol. 39, No. 12. pp. 2769-2776.
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