Estimation of the minimum effective dose of dietary supplement crocetin for prevention of myopia progression in mice

Kiwako Mori, Toshihide Kurihara, Xiaoyan Jiang, Shin Ichi Ikeda, Erisa Yotsukura, Hidemasa Torii, Kazuo Tsubota

Research output: Contribution to journalArticle

Abstract

The natural carotenoid crocetin has been reported to suppress phenotypes of an experimental myopia model in mice. We investigated the minimum effective dose to prevent myopia progression in a murine model. Three-week-old male mice (C57B6/J) were equipped with a −30 diopter (D) lens to induce myopia, and fed with normal chow, 0.0003%, or 0.001% of crocetin-containing chow. Changes in refractive errors and axial lengths (AL) were evaluated after three weeks. Pharmacokinetics of crocetin in the plasma and the eyeballs of mice was evaluated with specific high sensitivity quantitative analysis using liquid chromatography tandem mass spectrometry (LC-MS/MS) to determine the minimum effective dosage. A concentration of 0.001% of crocetin-containing chow showed a significant (p < 0.001) suppressive effect against both refractive and AL changes in the murine model. Meanwhile, there was no significant difference of AL change between the 0.0003% and the normal chow groups. The concentration of crocetin in the plasma and the eyeballs from mice fed with 0.001% crocetin-containing chow was significantly higher than control and 0.0003% crocetin-containing chow. In conclusion, we suggest 0.001% of crocetin-containing extract is the minimum effective dose showing a significant suppressive effect against both refractive and AL changes in the murine model.

Original languageEnglish
Article number180
JournalNutrients
Volume12
Issue number1
DOIs
Publication statusPublished - 2020 Jan 1

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Myopia
Dietary Supplements
dietary supplements
animal models
mice
dosage
Lens
liquid chromatography
pharmacokinetics
quantitative analysis
carotenoids
phenotype
Refractive Errors
myopia
crocetin
extracts
Carotenoids
Tandem Mass Spectrometry
Liquid Chromatography
Lenses

Keywords

  • Axial length
  • Crocetin
  • Minimum effective dose
  • Myopia
  • Myopia control
  • Refraction
  • Supplement

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

Cite this

Estimation of the minimum effective dose of dietary supplement crocetin for prevention of myopia progression in mice. / Mori, Kiwako; Kurihara, Toshihide; Jiang, Xiaoyan; Ikeda, Shin Ichi; Yotsukura, Erisa; Torii, Hidemasa; Tsubota, Kazuo.

In: Nutrients, Vol. 12, No. 1, 180, 01.01.2020.

Research output: Contribution to journalArticle

Mori, Kiwako ; Kurihara, Toshihide ; Jiang, Xiaoyan ; Ikeda, Shin Ichi ; Yotsukura, Erisa ; Torii, Hidemasa ; Tsubota, Kazuo. / Estimation of the minimum effective dose of dietary supplement crocetin for prevention of myopia progression in mice. In: Nutrients. 2020 ; Vol. 12, No. 1.
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