Estrogen inhibits the growth of MCF-7 cell variants resistant to transforming growth factor-beta

Yoshiaki Hagino, Masasumi Mawatari, Akihiko Yoshimura, Kimitoshi Kohno, Michio Kobayashi, Michihiko Kuwano

Research output: Contribution to journalArticle

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Abstract

Human breast cancer MCF-7 cells containing estrogen receptor are killed by transforming growth factor-beta (TGF-β). We isolated variants of MCF-7 highly resistant to TGF-β. Variants ES-1 and ES-4 were cloned, and the growth of ES-1 and ES-4 was found to be inhibited by estradiol, whereas estradiol stimulated the growth of the parental MCF-7 cells. ES-1 cells contained about 2-fold higher level of estradiol receptor than MCF-7 cells. Addition of estradiol to the culture medium for MCF-7 and the variant changed the expression of several secreted proteins. The repertoire of secreted proteins was markedly altered in the variant. Polypeptides of molecular weight 52,000 (52 K), 65 K and 160 K were increased about 10- to 50-fold in both estradiol-treated MCF-7 and ES-1 cells. Polypeptide of 130 K was decreased in estradiol-treated ES-1 cells while this polypeptide was increased about 4-fold in estradiol-treated MCF-7, as compared with untreated MCF-7. Polypeptide of 100 K was specifically secreted in ES-1 whether or not estradiol was present, but there appeared to be no significant amount of the 100 K protein in MCF-7. The estradiol-hypersensitive phenotype is discussed in relation to its aberrant expression of secreting proteins.

Original languageEnglish
Pages (from-to)74-81
Number of pages8
JournalJapanese Journal of Cancer Research
Volume79
Issue number1
Publication statusPublished - 1988 Jan
Externally publishedYes

Fingerprint

MCF-7 Cells
Transforming Growth Factor beta
Estradiol
Estrogens
Growth
Peptides
Estradiol Receptors
Proteins
Estrogen Receptors
Culture Media
Molecular Weight
Breast Neoplasms
Phenotype

Keywords

  • Estrogen effect
  • MCF-7 cell variants
  • TGF-β-resistance

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Estrogen inhibits the growth of MCF-7 cell variants resistant to transforming growth factor-beta. / Hagino, Yoshiaki; Mawatari, Masasumi; Yoshimura, Akihiko; Kohno, Kimitoshi; Kobayashi, Michio; Kuwano, Michihiko.

In: Japanese Journal of Cancer Research, Vol. 79, No. 1, 01.1988, p. 74-81.

Research output: Contribution to journalArticle

Hagino, Y, Mawatari, M, Yoshimura, A, Kohno, K, Kobayashi, M & Kuwano, M 1988, 'Estrogen inhibits the growth of MCF-7 cell variants resistant to transforming growth factor-beta', Japanese Journal of Cancer Research, vol. 79, no. 1, pp. 74-81.
Hagino, Yoshiaki ; Mawatari, Masasumi ; Yoshimura, Akihiko ; Kohno, Kimitoshi ; Kobayashi, Michio ; Kuwano, Michihiko. / Estrogen inhibits the growth of MCF-7 cell variants resistant to transforming growth factor-beta. In: Japanese Journal of Cancer Research. 1988 ; Vol. 79, No. 1. pp. 74-81.
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