Ethanol-induced leukocyte adherence and albumin leakage in rat mesenteric venules: Role of CD18/intercellular adhesion molecule-1

Masaaki Takaishi, Iwao Kurose, Hajime Higuchi, Naoyuki Watanabe, Tetsuya Nakamura, Shigeyuki Zeki, Jiro Nishida, Shinzo Kato, Soichiro Miura, Yoshio Mizuno, Peter R. Kvietys, D. Neil Granger, Hiromasa Ishii

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Abstract

The adherence and emigration of leukocytes have been implicated as a rate-limiting step in the microvascular disturbance in a variety of pathogenic events. The objective of the present study was to investigate leukocyte-endothelial cell adhesion and endothelial barrier function in rat mesenteric microvessels exposed to ethanol, which is known to cause inflammation and injury in various organs. Mesentery of male Wistar rats was used for intravital microscopic observations. Leukocyte adherence and albumin leakage were monitored in single postcapillary venules using the intravital fluorescence microscope. Superfusion of 50 mM ethanol elicited the leukocyte adherence and albumin leakage within 60 min. Pretreatment with a monoclonal antibody directed against either CD18 or intercellular adhesion molecule-1 (ICAM-1) significantly prevented the ethanol-induced increase in leukocyte adherence and decrease in barrier function of endothelium. These results suggest that ethanol-induced leukocyte adherence is mediated by CD18 on leukocytes end ICAM -1 on endothelial cells. The present study further supports that CD18/ICAM-1-dependent leukocyte-endothelial adhesive interactions lead to macromolecular leakage in the postcapillary venules exposed to ethanol.

Original languageEnglish
Pages (from-to)347A-349A
JournalAlcoholism: Clinical and Experimental Research
Volume20
Issue number9 SUPPL.
DOIs
Publication statusPublished - 1996 Dec 1

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Keywords

  • Albumin Leakage
  • CD18
  • ICAM-1
  • Leukocyte Adherence
  • Microcirculation

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology
  • Psychiatry and Mental health

Cite this

Takaishi, M., Kurose, I., Higuchi, H., Watanabe, N., Nakamura, T., Zeki, S., Nishida, J., Kato, S., Miura, S., Mizuno, Y., Kvietys, P. R., Granger, D. N., & Ishii, H. (1996). Ethanol-induced leukocyte adherence and albumin leakage in rat mesenteric venules: Role of CD18/intercellular adhesion molecule-1. Alcoholism: Clinical and Experimental Research, 20(9 SUPPL.), 347A-349A. https://doi.org/10.1111/j.1530-0277.1996.tb01170.x