Ethanol intake preceding Helicobacter pylori inoculation promotes gastric mucosal inflammation in Mongolian gerbils

Hidekazu Suzuki, Mikiji Mori, Koichi Seto, Shoichi Nagahashi, Chizuko Kawaguchi, Akemi Kai, Yasutada Akiba, Masayuki Suzuki, Makoto Suematsu, Soichiro Miura, Hiromasa Ishii

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Mongolian gerbils have been reported to be a suitable model for Helicobacter pylori-associated gastric mucosal injury, including gastric cancer. Although ethanol is known to be one of the harmful substances in the gastric mucosa, the relationship between ethanol and H. pylori infection remains unknown. The aim of the present study is to investigate the effect of ethanol treatment prior to H. pylori inoculation on associated gastric mucosal injury. Methods: Male Mongolian gerbils were used for the study. Helicobacter pylori was orally inoculated after 15 h fasting (Hp group). Thirty minutes prior to H. priori inoculation, a group of gerbils was orally treated with 40% ethanol (20 mL/kg; E + Hp group). Another group of animals was treated either with H. pylori culture media alone (controls) or with 40% ethanol plus culture media (E group). Gerbils were killed 2, 4 or 12 weeks after H. pylori inoculation. Helicobacter pylori infection was confirmed by both histological examination and serological tests. Mucosal damage was evaluated histologically according to the modified Sydney system. Results: Although in the controls and E group no significant change to the gastric mucose was observed, persistent H. pylori infection was seen in the mucosa and mucosal leucocyte infiltration and severe epithelial damage was observed in the Hp and E + Hp groups after 4 weeks. The histological scores for polymorphonuclear cell infiltration and myeloperoxidase activity were higher in the E + Hp group at 4 weeks than in the Hp group (P< 0.05). Conclusions: Ethanol intake preceding H. pylori inoculation could promote the progression of gastric mucosal inflammation in Mongolian gerbils.

Original languageEnglish
Pages (from-to)1062-1069
Number of pages8
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume14
Issue number11
DOIs
Publication statusPublished - 1999

Fingerprint

Gerbillinae
Helicobacter pylori
Stomach
Ethanol
Inflammation
Helicobacter Infections
Culture Media
Wounds and Injuries
Serologic Tests
Gastric Mucosa
Peroxidase
Stomach Neoplasms
Fasting
Mucous Membrane
Leukocytes
Control Groups

Keywords

  • Acute gastric mucosal lesions
  • Alcohol
  • Gastritis
  • Leucocyte infiltration

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

Cite this

Ethanol intake preceding Helicobacter pylori inoculation promotes gastric mucosal inflammation in Mongolian gerbils. / Suzuki, Hidekazu; Mori, Mikiji; Seto, Koichi; Nagahashi, Shoichi; Kawaguchi, Chizuko; Kai, Akemi; Akiba, Yasutada; Suzuki, Masayuki; Suematsu, Makoto; Miura, Soichiro; Ishii, Hiromasa.

In: Journal of Gastroenterology and Hepatology (Australia), Vol. 14, No. 11, 1999, p. 1062-1069.

Research output: Contribution to journalArticle

Suzuki, H, Mori, M, Seto, K, Nagahashi, S, Kawaguchi, C, Kai, A, Akiba, Y, Suzuki, M, Suematsu, M, Miura, S & Ishii, H 1999, 'Ethanol intake preceding Helicobacter pylori inoculation promotes gastric mucosal inflammation in Mongolian gerbils', Journal of Gastroenterology and Hepatology (Australia), vol. 14, no. 11, pp. 1062-1069. https://doi.org/10.1046/j.1440-1746.1999.01999.x
Suzuki, Hidekazu ; Mori, Mikiji ; Seto, Koichi ; Nagahashi, Shoichi ; Kawaguchi, Chizuko ; Kai, Akemi ; Akiba, Yasutada ; Suzuki, Masayuki ; Suematsu, Makoto ; Miura, Soichiro ; Ishii, Hiromasa. / Ethanol intake preceding Helicobacter pylori inoculation promotes gastric mucosal inflammation in Mongolian gerbils. In: Journal of Gastroenterology and Hepatology (Australia). 1999 ; Vol. 14, No. 11. pp. 1062-1069.
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abstract = "Background: Mongolian gerbils have been reported to be a suitable model for Helicobacter pylori-associated gastric mucosal injury, including gastric cancer. Although ethanol is known to be one of the harmful substances in the gastric mucosa, the relationship between ethanol and H. pylori infection remains unknown. The aim of the present study is to investigate the effect of ethanol treatment prior to H. pylori inoculation on associated gastric mucosal injury. Methods: Male Mongolian gerbils were used for the study. Helicobacter pylori was orally inoculated after 15 h fasting (Hp group). Thirty minutes prior to H. priori inoculation, a group of gerbils was orally treated with 40{\%} ethanol (20 mL/kg; E + Hp group). Another group of animals was treated either with H. pylori culture media alone (controls) or with 40{\%} ethanol plus culture media (E group). Gerbils were killed 2, 4 or 12 weeks after H. pylori inoculation. Helicobacter pylori infection was confirmed by both histological examination and serological tests. Mucosal damage was evaluated histologically according to the modified Sydney system. Results: Although in the controls and E group no significant change to the gastric mucose was observed, persistent H. pylori infection was seen in the mucosa and mucosal leucocyte infiltration and severe epithelial damage was observed in the Hp and E + Hp groups after 4 weeks. The histological scores for polymorphonuclear cell infiltration and myeloperoxidase activity were higher in the E + Hp group at 4 weeks than in the Hp group (P< 0.05). Conclusions: Ethanol intake preceding H. pylori inoculation could promote the progression of gastric mucosal inflammation in Mongolian gerbils.",
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T1 - Ethanol intake preceding Helicobacter pylori inoculation promotes gastric mucosal inflammation in Mongolian gerbils

AU - Suzuki, Hidekazu

AU - Mori, Mikiji

AU - Seto, Koichi

AU - Nagahashi, Shoichi

AU - Kawaguchi, Chizuko

AU - Kai, Akemi

AU - Akiba, Yasutada

AU - Suzuki, Masayuki

AU - Suematsu, Makoto

AU - Miura, Soichiro

AU - Ishii, Hiromasa

PY - 1999

Y1 - 1999

N2 - Background: Mongolian gerbils have been reported to be a suitable model for Helicobacter pylori-associated gastric mucosal injury, including gastric cancer. Although ethanol is known to be one of the harmful substances in the gastric mucosa, the relationship between ethanol and H. pylori infection remains unknown. The aim of the present study is to investigate the effect of ethanol treatment prior to H. pylori inoculation on associated gastric mucosal injury. Methods: Male Mongolian gerbils were used for the study. Helicobacter pylori was orally inoculated after 15 h fasting (Hp group). Thirty minutes prior to H. priori inoculation, a group of gerbils was orally treated with 40% ethanol (20 mL/kg; E + Hp group). Another group of animals was treated either with H. pylori culture media alone (controls) or with 40% ethanol plus culture media (E group). Gerbils were killed 2, 4 or 12 weeks after H. pylori inoculation. Helicobacter pylori infection was confirmed by both histological examination and serological tests. Mucosal damage was evaluated histologically according to the modified Sydney system. Results: Although in the controls and E group no significant change to the gastric mucose was observed, persistent H. pylori infection was seen in the mucosa and mucosal leucocyte infiltration and severe epithelial damage was observed in the Hp and E + Hp groups after 4 weeks. The histological scores for polymorphonuclear cell infiltration and myeloperoxidase activity were higher in the E + Hp group at 4 weeks than in the Hp group (P< 0.05). Conclusions: Ethanol intake preceding H. pylori inoculation could promote the progression of gastric mucosal inflammation in Mongolian gerbils.

AB - Background: Mongolian gerbils have been reported to be a suitable model for Helicobacter pylori-associated gastric mucosal injury, including gastric cancer. Although ethanol is known to be one of the harmful substances in the gastric mucosa, the relationship between ethanol and H. pylori infection remains unknown. The aim of the present study is to investigate the effect of ethanol treatment prior to H. pylori inoculation on associated gastric mucosal injury. Methods: Male Mongolian gerbils were used for the study. Helicobacter pylori was orally inoculated after 15 h fasting (Hp group). Thirty minutes prior to H. priori inoculation, a group of gerbils was orally treated with 40% ethanol (20 mL/kg; E + Hp group). Another group of animals was treated either with H. pylori culture media alone (controls) or with 40% ethanol plus culture media (E group). Gerbils were killed 2, 4 or 12 weeks after H. pylori inoculation. Helicobacter pylori infection was confirmed by both histological examination and serological tests. Mucosal damage was evaluated histologically according to the modified Sydney system. Results: Although in the controls and E group no significant change to the gastric mucose was observed, persistent H. pylori infection was seen in the mucosa and mucosal leucocyte infiltration and severe epithelial damage was observed in the Hp and E + Hp groups after 4 weeks. The histological scores for polymorphonuclear cell infiltration and myeloperoxidase activity were higher in the E + Hp group at 4 weeks than in the Hp group (P< 0.05). Conclusions: Ethanol intake preceding H. pylori inoculation could promote the progression of gastric mucosal inflammation in Mongolian gerbils.

KW - Acute gastric mucosal lesions

KW - Alcohol

KW - Gastritis

KW - Leucocyte infiltration

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