Ets-mediated brain induction in embryos of the ascidian Halocynthia roretzi

Takashi Akanuma, Hiroki Nishida

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The larval ascidian brain (sensory vesicle) is located on the dorsal side of the trunk region and forms part of the anterior central nervous system. Sensory organs such as the otolith, ocellus, and hydrostatic-pressure organ reside in the brain. The brain coordinates the core roles of the larval nervous system. The brain is derived from anterior animal a-line blastomeres. The default fate of these blastomeres is epidermis, and the inductive signals from anterior vegetal blastomeres convert the fate into brain. It remains unclear, however, when these inductive interactions take place. To determine when, we examined whether partial embryos derived from brain-lineage blastomeres isolated at various stages express neural and epidermal marker genes. Partial embryos derived from brain-lineage blastomeres isolated after the 32-cell stage expressed all the neural marker genes examined. The expression of the epidermal marker gene was first reduced in partial embryos when blastomeres were isolated at the 64-cell stage. Moreover, the process for brain specification seemed to continue after the 110-cell stage. We also investigated the function of HrEts, an ascidian homolog of Ets transcription factors, to elucidate the molecular mechanism of brain induction. HrEts functions were inhibited by the use of antisense morpholino oligonucleotides. Loss of Ets functions resulted in loss of the expression of some of the neural marker genes and the ectopic expression of the epidermal marker gene in brain precursor cells. These results suggest that HrEts is an essential transcription factor that mediates ascidian brain induction.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalDevelopment Genes and Evolution
Volume214
Issue number1
DOIs
Publication statusPublished - 2004 Jan
Externally publishedYes

Fingerprint

Halocynthia roretzi
Urochordata
Ascidiacea
brain
embryo
embryo (animal)
Embryonic Structures
Blastomeres
blastomeres
Brain
genetic markers
gene
nervous system
Genes
transcription factors
Proto-Oncogene Proteins c-ets
cells
Otolithic Membrane
ocelli
Morpholinos

Keywords

  • Ascidian embryogenesis
  • Brain
  • Developmental fate
  • Ets
  • Neural induction

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Genetics
  • Ecology, Evolution, Behavior and Systematics

Cite this

Ets-mediated brain induction in embryos of the ascidian Halocynthia roretzi. / Akanuma, Takashi; Nishida, Hiroki.

In: Development Genes and Evolution, Vol. 214, No. 1, 01.2004, p. 1-9.

Research output: Contribution to journalArticle

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