Evaluation of antibiotic-loaded calcium phosphate bone cement in a cranium-infected experimental model

Yoshiaki Sakamoto, Hiroko Ochiai, Ikuko Ohsugi, Yoshikazu Inoue, Yoko Yoshimura, Kazuo Kishi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Treatment of calvarial defects has remained a challenge in reconstruction surgery, especially because of infection at these sites. We produced a bactericidal biomaterial for treating infected bone defects by using calcium phosphate bone cement mixed with antibiotics. We evaluated the usefulness of this material mixed with the antibiotic vancomycin in a cranium-infected rat model. The concentration of vancomycin used was 5.0 wt%, as reported in our previous study. In order to establish the rat model, a cranium defect (diameter, 5 mm) was made that was infected with methicillin-resistant Staphylococcus aureus (MRSA). Thirty-six rats were divided into 6 groups depending on whether an autologous graft or bone cement with or without antibiotic was used for the defect. After 1 and 4 weeks, abscess formation was checked, tissue bacterial counts were determined, and pathological examination was performed. At both 1 and 4 weeks, no MRSA was detected on tissue bacterial culture or pathological examination in groups that received bone cement with antibiotics. In groups that received bone cement without antibiotic, MRSA was detected, and the bone cement had compromised and disintegrated into several slices. In conclusion, bone cement that contains antibiotics appears to be effective not only for reconstruction in cases of cranial defect, but also in terms of preventing infection.

Original languageEnglish
Pages (from-to)647-653
Number of pages7
JournalNeurologia Medico-Chirurgica
Volume54
Issue number8
DOIs
Publication statusPublished - 2014

Fingerprint

Bone Cements
Skull
Theoretical Models
Anti-Bacterial Agents
Methicillin-Resistant Staphylococcus aureus
Vancomycin
Bacterial Load
Biocompatible Materials
Infection
Abscess
calcium phosphate
Transplants
Bone and Bones

Keywords

  • Antibiotics
  • Calcium phosphate cement
  • Hydroxyapatite
  • Methicillin-resistant Staphylococcus aureus (MRSA)

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

Cite this

Evaluation of antibiotic-loaded calcium phosphate bone cement in a cranium-infected experimental model. / Sakamoto, Yoshiaki; Ochiai, Hiroko; Ohsugi, Ikuko; Inoue, Yoshikazu; Yoshimura, Yoko; Kishi, Kazuo.

In: Neurologia Medico-Chirurgica, Vol. 54, No. 8, 2014, p. 647-653.

Research output: Contribution to journalArticle

Sakamoto, Yoshiaki ; Ochiai, Hiroko ; Ohsugi, Ikuko ; Inoue, Yoshikazu ; Yoshimura, Yoko ; Kishi, Kazuo. / Evaluation of antibiotic-loaded calcium phosphate bone cement in a cranium-infected experimental model. In: Neurologia Medico-Chirurgica. 2014 ; Vol. 54, No. 8. pp. 647-653.
@article{38a7a8be0a6641bf8fec9226b4c4a172,
title = "Evaluation of antibiotic-loaded calcium phosphate bone cement in a cranium-infected experimental model",
abstract = "Treatment of calvarial defects has remained a challenge in reconstruction surgery, especially because of infection at these sites. We produced a bactericidal biomaterial for treating infected bone defects by using calcium phosphate bone cement mixed with antibiotics. We evaluated the usefulness of this material mixed with the antibiotic vancomycin in a cranium-infected rat model. The concentration of vancomycin used was 5.0 wt{\%}, as reported in our previous study. In order to establish the rat model, a cranium defect (diameter, 5 mm) was made that was infected with methicillin-resistant Staphylococcus aureus (MRSA). Thirty-six rats were divided into 6 groups depending on whether an autologous graft or bone cement with or without antibiotic was used for the defect. After 1 and 4 weeks, abscess formation was checked, tissue bacterial counts were determined, and pathological examination was performed. At both 1 and 4 weeks, no MRSA was detected on tissue bacterial culture or pathological examination in groups that received bone cement with antibiotics. In groups that received bone cement without antibiotic, MRSA was detected, and the bone cement had compromised and disintegrated into several slices. In conclusion, bone cement that contains antibiotics appears to be effective not only for reconstruction in cases of cranial defect, but also in terms of preventing infection.",
keywords = "Antibiotics, Calcium phosphate cement, Hydroxyapatite, Methicillin-resistant Staphylococcus aureus (MRSA)",
author = "Yoshiaki Sakamoto and Hiroko Ochiai and Ikuko Ohsugi and Yoshikazu Inoue and Yoko Yoshimura and Kazuo Kishi",
year = "2014",
doi = "10.2176/nmc.oa.2013-0295",
language = "English",
volume = "54",
pages = "647--653",
journal = "Neurologia Medico-Chirurgica",
issn = "0470-8105",
publisher = "Japan Neurosurgical Society",
number = "8",

}

TY - JOUR

T1 - Evaluation of antibiotic-loaded calcium phosphate bone cement in a cranium-infected experimental model

AU - Sakamoto, Yoshiaki

AU - Ochiai, Hiroko

AU - Ohsugi, Ikuko

AU - Inoue, Yoshikazu

AU - Yoshimura, Yoko

AU - Kishi, Kazuo

PY - 2014

Y1 - 2014

N2 - Treatment of calvarial defects has remained a challenge in reconstruction surgery, especially because of infection at these sites. We produced a bactericidal biomaterial for treating infected bone defects by using calcium phosphate bone cement mixed with antibiotics. We evaluated the usefulness of this material mixed with the antibiotic vancomycin in a cranium-infected rat model. The concentration of vancomycin used was 5.0 wt%, as reported in our previous study. In order to establish the rat model, a cranium defect (diameter, 5 mm) was made that was infected with methicillin-resistant Staphylococcus aureus (MRSA). Thirty-six rats were divided into 6 groups depending on whether an autologous graft or bone cement with or without antibiotic was used for the defect. After 1 and 4 weeks, abscess formation was checked, tissue bacterial counts were determined, and pathological examination was performed. At both 1 and 4 weeks, no MRSA was detected on tissue bacterial culture or pathological examination in groups that received bone cement with antibiotics. In groups that received bone cement without antibiotic, MRSA was detected, and the bone cement had compromised and disintegrated into several slices. In conclusion, bone cement that contains antibiotics appears to be effective not only for reconstruction in cases of cranial defect, but also in terms of preventing infection.

AB - Treatment of calvarial defects has remained a challenge in reconstruction surgery, especially because of infection at these sites. We produced a bactericidal biomaterial for treating infected bone defects by using calcium phosphate bone cement mixed with antibiotics. We evaluated the usefulness of this material mixed with the antibiotic vancomycin in a cranium-infected rat model. The concentration of vancomycin used was 5.0 wt%, as reported in our previous study. In order to establish the rat model, a cranium defect (diameter, 5 mm) was made that was infected with methicillin-resistant Staphylococcus aureus (MRSA). Thirty-six rats were divided into 6 groups depending on whether an autologous graft or bone cement with or without antibiotic was used for the defect. After 1 and 4 weeks, abscess formation was checked, tissue bacterial counts were determined, and pathological examination was performed. At both 1 and 4 weeks, no MRSA was detected on tissue bacterial culture or pathological examination in groups that received bone cement with antibiotics. In groups that received bone cement without antibiotic, MRSA was detected, and the bone cement had compromised and disintegrated into several slices. In conclusion, bone cement that contains antibiotics appears to be effective not only for reconstruction in cases of cranial defect, but also in terms of preventing infection.

KW - Antibiotics

KW - Calcium phosphate cement

KW - Hydroxyapatite

KW - Methicillin-resistant Staphylococcus aureus (MRSA)

UR - http://www.scopus.com/inward/record.url?scp=84906809894&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84906809894&partnerID=8YFLogxK

U2 - 10.2176/nmc.oa.2013-0295

DO - 10.2176/nmc.oa.2013-0295

M3 - Article

VL - 54

SP - 647

EP - 653

JO - Neurologia Medico-Chirurgica

JF - Neurologia Medico-Chirurgica

SN - 0470-8105

IS - 8

ER -