Evaluation of autologous cultured epithelium as replacement skin after tattoo excision: Correlation between skin texture and histological features

K. Kadoya, S. Amano, S. Inomata, M. Tsunenaga, K. Matsuzaki, H. Oshima, M. Tanabe, N. Kumagai, Toshio Nishiyama

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Cultured epidermal autographs (CEAs) are currently used as a coverage treatment for burn wounds, for disfiguring burn scars involving depigmentation and in restoring the elasticity of the skin. The advantage of CEAs is that epidermal sheets prepared from small skin pieces can be enlarged sufficiently to cover large burn areas. Objectives: We examined the correlation between recovery of skin texture, and elastic fibre formation and keratinocyte differentiation (assessed by immunohistochemistry) in CEAs used as replacement skin after tattoo excision in a Japanese patient. Methods: The tattooed skin was excised down to the deep dermal layer and then CEA was transplanted onto the patient. The skin textures were evaluated by taking replicas of the skin surface, and histological changes of filaggrin, transglutaminase, involucrin, fibrillin and elastin in the autograft skin were examined by immunohistochemistry. Results: The skin texture improved with time after grafting the CEA, and appeared similar to that of normal skin at 39 months. Among keratinocyte differentiation markers, filaggrin recovered to a normal pattern at around 6 months, and transglutaminase did so at 39 months, whereas involucrin expression remained abnormal at 39 months. Fibrillin expression appeared similar to that of normal skin by 39 months, except for sparse candelabra-like structures of short fibres. Elastin expression remained at a low level throughout. Conclusions: Our results show that the recovery of skin texture after application of CEAs following tattoo excision is associated with the normalization of epidermal differentiation markers, except involucrin, and with the regeneration of elastic fibres in the dermis.

Original languageEnglish
Pages (from-to)377-380
Number of pages4
JournalBritish Journal of Dermatology
Volume149
Issue number2
DOIs
Publication statusPublished - 2003 Sep 1
Externally publishedYes

Fingerprint

Epithelium
Skin
Transglutaminases
Elastic Tissue
Elastin
Differentiation Antigens
Keratinocytes
Immunohistochemistry
Elasticity
Autografts
Dermis
Cicatrix
Regeneration
Wounds and Injuries

Keywords

  • Cultured epidermal autografts
  • Elastic fibres
  • Keratinocyte differentiation markers
  • Skin texture
  • Tattoo

ASJC Scopus subject areas

  • Dermatology

Cite this

Evaluation of autologous cultured epithelium as replacement skin after tattoo excision : Correlation between skin texture and histological features. / Kadoya, K.; Amano, S.; Inomata, S.; Tsunenaga, M.; Matsuzaki, K.; Oshima, H.; Tanabe, M.; Kumagai, N.; Nishiyama, Toshio.

In: British Journal of Dermatology, Vol. 149, No. 2, 01.09.2003, p. 377-380.

Research output: Contribution to journalArticle

Kadoya, K, Amano, S, Inomata, S, Tsunenaga, M, Matsuzaki, K, Oshima, H, Tanabe, M, Kumagai, N & Nishiyama, T 2003, 'Evaluation of autologous cultured epithelium as replacement skin after tattoo excision: Correlation between skin texture and histological features', British Journal of Dermatology, vol. 149, no. 2, pp. 377-380. https://doi.org/10.1046/j.1365-2133.2003.05382.x
Kadoya, K. ; Amano, S. ; Inomata, S. ; Tsunenaga, M. ; Matsuzaki, K. ; Oshima, H. ; Tanabe, M. ; Kumagai, N. ; Nishiyama, Toshio. / Evaluation of autologous cultured epithelium as replacement skin after tattoo excision : Correlation between skin texture and histological features. In: British Journal of Dermatology. 2003 ; Vol. 149, No. 2. pp. 377-380.
@article{6a2e0aee63614c379f0085538b498482,
title = "Evaluation of autologous cultured epithelium as replacement skin after tattoo excision: Correlation between skin texture and histological features",
abstract = "Background: Cultured epidermal autographs (CEAs) are currently used as a coverage treatment for burn wounds, for disfiguring burn scars involving depigmentation and in restoring the elasticity of the skin. The advantage of CEAs is that epidermal sheets prepared from small skin pieces can be enlarged sufficiently to cover large burn areas. Objectives: We examined the correlation between recovery of skin texture, and elastic fibre formation and keratinocyte differentiation (assessed by immunohistochemistry) in CEAs used as replacement skin after tattoo excision in a Japanese patient. Methods: The tattooed skin was excised down to the deep dermal layer and then CEA was transplanted onto the patient. The skin textures were evaluated by taking replicas of the skin surface, and histological changes of filaggrin, transglutaminase, involucrin, fibrillin and elastin in the autograft skin were examined by immunohistochemistry. Results: The skin texture improved with time after grafting the CEA, and appeared similar to that of normal skin at 39 months. Among keratinocyte differentiation markers, filaggrin recovered to a normal pattern at around 6 months, and transglutaminase did so at 39 months, whereas involucrin expression remained abnormal at 39 months. Fibrillin expression appeared similar to that of normal skin by 39 months, except for sparse candelabra-like structures of short fibres. Elastin expression remained at a low level throughout. Conclusions: Our results show that the recovery of skin texture after application of CEAs following tattoo excision is associated with the normalization of epidermal differentiation markers, except involucrin, and with the regeneration of elastic fibres in the dermis.",
keywords = "Cultured epidermal autografts, Elastic fibres, Keratinocyte differentiation markers, Skin texture, Tattoo",
author = "K. Kadoya and S. Amano and S. Inomata and M. Tsunenaga and K. Matsuzaki and H. Oshima and M. Tanabe and N. Kumagai and Toshio Nishiyama",
year = "2003",
month = "9",
day = "1",
doi = "10.1046/j.1365-2133.2003.05382.x",
language = "English",
volume = "149",
pages = "377--380",
journal = "British Journal of Dermatology",
issn = "0007-0963",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Evaluation of autologous cultured epithelium as replacement skin after tattoo excision

T2 - Correlation between skin texture and histological features

AU - Kadoya, K.

AU - Amano, S.

AU - Inomata, S.

AU - Tsunenaga, M.

AU - Matsuzaki, K.

AU - Oshima, H.

AU - Tanabe, M.

AU - Kumagai, N.

AU - Nishiyama, Toshio

PY - 2003/9/1

Y1 - 2003/9/1

N2 - Background: Cultured epidermal autographs (CEAs) are currently used as a coverage treatment for burn wounds, for disfiguring burn scars involving depigmentation and in restoring the elasticity of the skin. The advantage of CEAs is that epidermal sheets prepared from small skin pieces can be enlarged sufficiently to cover large burn areas. Objectives: We examined the correlation between recovery of skin texture, and elastic fibre formation and keratinocyte differentiation (assessed by immunohistochemistry) in CEAs used as replacement skin after tattoo excision in a Japanese patient. Methods: The tattooed skin was excised down to the deep dermal layer and then CEA was transplanted onto the patient. The skin textures were evaluated by taking replicas of the skin surface, and histological changes of filaggrin, transglutaminase, involucrin, fibrillin and elastin in the autograft skin were examined by immunohistochemistry. Results: The skin texture improved with time after grafting the CEA, and appeared similar to that of normal skin at 39 months. Among keratinocyte differentiation markers, filaggrin recovered to a normal pattern at around 6 months, and transglutaminase did so at 39 months, whereas involucrin expression remained abnormal at 39 months. Fibrillin expression appeared similar to that of normal skin by 39 months, except for sparse candelabra-like structures of short fibres. Elastin expression remained at a low level throughout. Conclusions: Our results show that the recovery of skin texture after application of CEAs following tattoo excision is associated with the normalization of epidermal differentiation markers, except involucrin, and with the regeneration of elastic fibres in the dermis.

AB - Background: Cultured epidermal autographs (CEAs) are currently used as a coverage treatment for burn wounds, for disfiguring burn scars involving depigmentation and in restoring the elasticity of the skin. The advantage of CEAs is that epidermal sheets prepared from small skin pieces can be enlarged sufficiently to cover large burn areas. Objectives: We examined the correlation between recovery of skin texture, and elastic fibre formation and keratinocyte differentiation (assessed by immunohistochemistry) in CEAs used as replacement skin after tattoo excision in a Japanese patient. Methods: The tattooed skin was excised down to the deep dermal layer and then CEA was transplanted onto the patient. The skin textures were evaluated by taking replicas of the skin surface, and histological changes of filaggrin, transglutaminase, involucrin, fibrillin and elastin in the autograft skin were examined by immunohistochemistry. Results: The skin texture improved with time after grafting the CEA, and appeared similar to that of normal skin at 39 months. Among keratinocyte differentiation markers, filaggrin recovered to a normal pattern at around 6 months, and transglutaminase did so at 39 months, whereas involucrin expression remained abnormal at 39 months. Fibrillin expression appeared similar to that of normal skin by 39 months, except for sparse candelabra-like structures of short fibres. Elastin expression remained at a low level throughout. Conclusions: Our results show that the recovery of skin texture after application of CEAs following tattoo excision is associated with the normalization of epidermal differentiation markers, except involucrin, and with the regeneration of elastic fibres in the dermis.

KW - Cultured epidermal autografts

KW - Elastic fibres

KW - Keratinocyte differentiation markers

KW - Skin texture

KW - Tattoo

UR - http://www.scopus.com/inward/record.url?scp=0042914410&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0042914410&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2133.2003.05382.x

DO - 10.1046/j.1365-2133.2003.05382.x

M3 - Article

C2 - 12932246

AN - SCOPUS:0042914410

VL - 149

SP - 377

EP - 380

JO - British Journal of Dermatology

JF - British Journal of Dermatology

SN - 0007-0963

IS - 2

ER -