Evaluation of bifidobacterial adhesion to acidic sugar chains of porcine colonic mucins

Keita Nishiyama, Akira Kawanabe, Hirofumi Miyauchi, Fumiaki Abe, Daigo Tsubokawa, Kazuhiko Ishihara, Yuji Yamamoto, Takao Mukai

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The aim of this study was to assess the adhesion of Bifidobacterium strains to acidic carbohydrate moieties of porcine colonic mucin. Mucins were extracted and purified via gel filtration chromatography followed by density-gradient ultracentrifugation. The presence of sulfated and sialylated carbohydrates in mucins was shown by enzymelinked immunosorbent assays using PGM34 and HMC31 monoclonal antibodies (mAbs), respectively. Adhesion of Bifidobacterium strains to mucin preparations was markedly affected by the degree of purification. In eight of 22 strains, we observed increased adhesion to mucin preparations purified by ultracentrifugation. Moreover, in some of these eight strains, adhesion to mucin was reduced by pretreatment with sulfatase and/or sialidase, and competitively inhibited by pretreatment with PGM34 and/or HCM31 mAbs. Our results showed that some Bifidobacterium strains adhered to sulfoand/or sialomucin and were able to recognize carbohydrate structures of the mAbs epitopes.

Original languageEnglish
Pages (from-to)1444-1451
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume78
Issue number8
DOIs
Publication statusPublished - 2014 Jan 1
Externally publishedYes

Fingerprint

Mucins
Sugars
Swine
Adhesion
Bifidobacterium
Ultracentrifugation
Monoclonal Antibodies
Carbohydrates
Sialomucins
Sulfatases
Immunosorbents
Neuraminidase
Chromatography
Purification
Gel Chromatography
Epitopes
Assays
Gels

Keywords

  • Acidic mucin
  • Adherence
  • Bifidobacterium
  • Probiotics
  • Sugar chain

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Evaluation of bifidobacterial adhesion to acidic sugar chains of porcine colonic mucins. / Nishiyama, Keita; Kawanabe, Akira; Miyauchi, Hirofumi; Abe, Fumiaki; Tsubokawa, Daigo; Ishihara, Kazuhiko; Yamamoto, Yuji; Mukai, Takao.

In: Bioscience, Biotechnology and Biochemistry, Vol. 78, No. 8, 01.01.2014, p. 1444-1451.

Research output: Contribution to journalArticle

Nishiyama, K, Kawanabe, A, Miyauchi, H, Abe, F, Tsubokawa, D, Ishihara, K, Yamamoto, Y & Mukai, T 2014, 'Evaluation of bifidobacterial adhesion to acidic sugar chains of porcine colonic mucins', Bioscience, Biotechnology and Biochemistry, vol. 78, no. 8, pp. 1444-1451. https://doi.org/10.1080/09168451.2014.918491
Nishiyama, Keita ; Kawanabe, Akira ; Miyauchi, Hirofumi ; Abe, Fumiaki ; Tsubokawa, Daigo ; Ishihara, Kazuhiko ; Yamamoto, Yuji ; Mukai, Takao. / Evaluation of bifidobacterial adhesion to acidic sugar chains of porcine colonic mucins. In: Bioscience, Biotechnology and Biochemistry. 2014 ; Vol. 78, No. 8. pp. 1444-1451.
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