Evaluation of conjunctival inflammatory status by confocal scanning laser microscopy and conjunctival brush cytology in patients with atopic keratoconjunctivitis (AKC)

Tais Hitomi Wakamatsu, Naoko Okada, Takashi Kojima, Yukihiro Matsumoto, Osama M A Ibrahim, Murat Dogru, Enrique Sato Adan, Kazumi Fukagawa, Chikako Katakami, Kazuo Tsubota, Jun Shimazaki, Hiroshi Fujishima

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Purpose: To elucidate the status of the conjunctival inflammation in atopic keratoconjunctivitis (AKC) using laser scanning confocal microscopy and compare the relevant findings with conjunctival brush cytology in a prospective controlled study. Methods: Twenty eyes from 20 AKC patients as well as 16 eyes from 16 age and sex matched normal subjects were studied. The subjects underwent tear film break-up time (BUT), fluorescein and Rose Bengal staining of the ocular surface, conjunctival confocal microscopy, Schirmer test, and brush cytology. Brush cytology specimens and in vivo confocal microscopy scans underwent evaluation for inflammatory cell densities. Results: Brush cytology specimens and in vivo confocal microscopy scans from AKC patients revealed significantly higher numbers of inflammatory cells (p<0.05). Conjunctival inflammatory cell density showed a negative correlation with tear stability and a positive correlation with vital staining scores and conjunctival injection grades. The extent of conjunctival inflammation assessed by in vivo confocal microscopy showed a strong positive linear correlation with the inflammation status evaluated by brush cytology. The corneal inflammatory cell density assessed by in vivo confocal microscopy showed a significant negative correlation with tear stability and a positive linear correlation with corneal fluorescein staining. Conclusions: Confocal scanning laser microscopy is an efficient, noninvasive, and a promising tool for the quantitative assessment of conjunctival inflammation, a parameter of this new technology which correlated well with subjective and objective ocular surface clinical findings.

Original languageEnglish
Pages (from-to)1611-1619
Number of pages9
JournalMolecular Vision
Volume15
Publication statusPublished - 2009 Aug 15

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Keratoconjunctivitis
Confocal Microscopy
Cell Biology
Cell Count
Tears
Inflammation
Staining and Labeling
Fluorescein
Rose Bengal
Prospective Studies
Technology
Injections
Intravital Microscopy

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Wakamatsu, T. H., Okada, N., Kojima, T., Matsumoto, Y., Ibrahim, O. M. A., Dogru, M., ... Fujishima, H. (2009). Evaluation of conjunctival inflammatory status by confocal scanning laser microscopy and conjunctival brush cytology in patients with atopic keratoconjunctivitis (AKC). Molecular Vision, 15, 1611-1619.

Evaluation of conjunctival inflammatory status by confocal scanning laser microscopy and conjunctival brush cytology in patients with atopic keratoconjunctivitis (AKC). / Wakamatsu, Tais Hitomi; Okada, Naoko; Kojima, Takashi; Matsumoto, Yukihiro; Ibrahim, Osama M A; Dogru, Murat; Adan, Enrique Sato; Fukagawa, Kazumi; Katakami, Chikako; Tsubota, Kazuo; Shimazaki, Jun; Fujishima, Hiroshi.

In: Molecular Vision, Vol. 15, 15.08.2009, p. 1611-1619.

Research output: Contribution to journalArticle

Wakamatsu, TH, Okada, N, Kojima, T, Matsumoto, Y, Ibrahim, OMA, Dogru, M, Adan, ES, Fukagawa, K, Katakami, C, Tsubota, K, Shimazaki, J & Fujishima, H 2009, 'Evaluation of conjunctival inflammatory status by confocal scanning laser microscopy and conjunctival brush cytology in patients with atopic keratoconjunctivitis (AKC)', Molecular Vision, vol. 15, pp. 1611-1619.
Wakamatsu, Tais Hitomi ; Okada, Naoko ; Kojima, Takashi ; Matsumoto, Yukihiro ; Ibrahim, Osama M A ; Dogru, Murat ; Adan, Enrique Sato ; Fukagawa, Kazumi ; Katakami, Chikako ; Tsubota, Kazuo ; Shimazaki, Jun ; Fujishima, Hiroshi. / Evaluation of conjunctival inflammatory status by confocal scanning laser microscopy and conjunctival brush cytology in patients with atopic keratoconjunctivitis (AKC). In: Molecular Vision. 2009 ; Vol. 15. pp. 1611-1619.
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