Evaluation of electrical propagation delay with cardiomyocytes by photosensitization reaction in vitro

Marika Doi, Emiyu Ogawa, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In order to study cardiomyocyte electrical conduction damage by a photosensitization reaction (PR) mostly comes from outside of the cells in a few minutes after the PR, we studied propagation delay of contact action potential with cardiomyocyte by the PR. To determine appropriate PR condition for tachyarrhythmia ablation, a precise electrophysiological experiment in vitro has been preferable. We measured the contact action potential using a microelectrode array system of which information may be correct than conventional Ca2+ measurement. We investigated the propagation delays of an evoked potential to evaluate the electrical conduction damage by the PR. Rat cardiomyocytes were cultivated for 5-7 days on a dish with which 64 electrodes were patterned, in an incubator controlled to 37°C, 5% CO2. The following conditions were used for the PR: 40 μg/ml talapordfin sodium and 290 mW/cm2, 40-78 J/cm2 for an irradiation. A 2D map was obtained to visualize the propagation delays of the evoked potential. The propagation speed, which was calculated based on the measured propagation delays, was decreased by about 30-50% on average of all electrodes after the PR. Therefore, we think 2D propagation delays measurement of the evoked potential with contact action potential measuring system might be available to evaluate the acute electrical conduction damage of cardiomyocyte by the PR.

Original languageEnglish
Title of host publicationOptical Interactions with Tissue and Cells XXVIII
PublisherSPIE
Volume10062
ISBN (Electronic)9781510605657
DOIs
Publication statusPublished - 2017
EventOptical Interactions with Tissue and Cells XXVIII - San Francisco, United States
Duration: 2017 Jan 302017 Jan 31

Other

OtherOptical Interactions with Tissue and Cells XXVIII
CountryUnited States
CitySan Francisco
Period17/1/3017/1/31

Keywords

  • acute electrical conduction damage
  • cardiomyocyte
  • extracellular potential measurement
  • photosensitization reaction

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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