Evaluation of human conjunctival epithelium by a combination of brush cytology and flow cytometry

An approach to the quantitative technique

Tsutomu Fujihara, Tsutomu Takeuchi, Keiko Saito, Yasuo Kitajima, Tadao K. Kobayashi, Kazuo Tsubota

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Cytology using the brush technique is readily available and is a rapid means of establishing a presumptive diagnosis of ocular surface changes. However, those techniques have some limitations when obtaining cells from certain localized areas particularly if using the Cytobrush-S®. We have described here a new type of brush (Accellon-M®), which can collect the local cells using its spherical tip, and evaluated them by comparing with Cytobrush-S®. Furthermore, we differentiated epithelial cells from non- epithelial cells that were collected by brushing, and by a combination of brush cytology and flow cytometry using an anti-keratin antibody, AE-3, which reacts with all basic epithelial keratins. Accellon-M® could collect the epithelial cells from conjunctiva as effectively as with the Cytobrush-S®, and there were no statistical differences between both groups. AE-3, which is reported as the marker of epithelial cells, were detected quantitatively by a combination of brush cytology and flow cytometry techniques. The result of the present study emphasizes that both the Cytobrush-S® and the Accellon- M® are valuable for conjunctival brush cytology. An additional positive feature of the Accellon-M® may be improved cell collection from the conjunctival epithelium especially when the target cells are in a limited area. The combination of the brush cytology and the flow cytometry technique appears to be a useful adjunct as an additional diagnostic or research tool for use in the detection of various antigens in the conjunctival epithelium.

Original languageEnglish
Pages (from-to)456-460
Number of pages5
JournalDiagnostic Cytopathology
Volume17
Issue number6
DOIs
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Cell Biology
Flow Cytometry
Epithelium
Epithelial Cells
Type II Keratin
Conjunctiva
Keratins
Anti-Idiotypic Antibodies
Antigens
Research

Keywords

  • Accellon-M®
  • Brush cytology
  • Conjunctival epithelial cells
  • Cytobrush-S®
  • Flow cytometry
  • Immunofluorescence

ASJC Scopus subject areas

  • Anatomy

Cite this

Evaluation of human conjunctival epithelium by a combination of brush cytology and flow cytometry : An approach to the quantitative technique. / Fujihara, Tsutomu; Takeuchi, Tsutomu; Saito, Keiko; Kitajima, Yasuo; Kobayashi, Tadao K.; Tsubota, Kazuo.

In: Diagnostic Cytopathology, Vol. 17, No. 6, 1997, p. 456-460.

Research output: Contribution to journalArticle

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