Evaluation of lipid accumulation using electrical impedance measurement under three-dimensional culture condition

Daiki Zemmyo, Shogo Miyata

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The degeneration of adipocyte has been reported to cause obesity, metabolic syndrome, and other diseases. To treat these diseases, an effective in vitro evaluation and drug-screening system for adipocyte culture is required. The objective of this study is to establish an in vitro three-dimensional cell culture system to enable the monitoring of lipid accumulation by measuring electrical impedance, and to determine the relationship between the impedance and lipid accumulation of adipocytes cultured three dimensionally. Consequently, pre-adipocytes, 3T3-L1 cells, were cultured and differentiated to the adipocytes in our culture system, and the electrical impedance of the three-dimensional adipocyte culture at a high frequency was related to the lipid accumulation of the adipocytes. In conclusion, the lipid accumulation of adipocytes could be evaluated in real time by monitoring the electrical impedance during in vitro culture.

Original languageEnglish
Article number455
JournalMicromachines
Volume10
Issue number7
DOIs
Publication statusPublished - 2019 Jul 1

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Keywords

  • 3T3-L1
  • Adipocyte
  • Electrical impedance measurement
  • Lipid droplet
  • Three-dimensional cell culture

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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