Evaluation of lipid oxidative stress status in Sjögren syndrome patients

Tais H. Wakamatsu, Murat Dogru, Yukihiro Matsumoto, Takashi Kojima, Minako Kaido, Osama M A Ibrahim, Enrique A. Sato, Ayako Igarashi, Yoshiyuki Ichihashi, Yoshiyuki Satake, Jun Shimazaki, Kazuo Tsubota

Research output: Contribution to journalArticle

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Abstract

PURPOSE. We evaluated the levels of lipid oxidative stress markers and inflammatory cells from tears and conjunctiva of patients with Sjögren syndrome (SS) and normal subjects. METHODS. We examined 31 eyes of 16 patients (16 females) with SS and 15 eyes of 10 healthy controls (2 males and 8 females) in this prospective study. All subjects underwent a Schirmer test, measurement of tear film break-up time, vital stainings, confocal microscopy of the conjunctiva, tear collection for hexanoyl-lysine (HEL), ELISA, and conjunctival brush cytology. Brush cytology samples underwent immunohistochemistry (IHC) staining with HEL and 4-hydroxy-2- nonenal (4HNE). Hematoxylin-eosin and IHC staining with HEL and 4HNE also were performed on conjunctival samples of SS patients and controls. RESULTS. The tear stability and vital staining scores were significantly worse in eyes of SS patients compared to the controls. Conjunctival inflammatory cell density was significantly higher in SS subjects compared to controls. The numbers of conjunctival cells stained positively for HEL and 4HNE were significantly higher in SS patients compared to controls. Tear HEL concentrations correlated significantly with staining scores and inflammatory cell density in confocal microscopy. Conjunctival specimens also revealed higher numbers of cells stained positively for inflammatory markers, as well as HEL and 4HNE in the IHC stainings. CONCLUSIONS. Increase of the oxidative stress status in the conjunctiva of SS patients appears to have a role in the pathogenesis of dry eye disease. A close relationship may exist between reactive oxygen species (ROS) production, lipid peroxidation related membrane damage, and inflammatory processes in dry eye.

Original languageEnglish
Pages (from-to)201-210
Number of pages10
JournalInvestigative Ophthalmology and Visual Science
Volume54
Issue number1
DOIs
Publication statusPublished - 2013 Jan

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Oxidative Stress
Lysine
Tears
Lipids
Staining and Labeling
Conjunctiva
Cell Count
Immunohistochemistry
Confocal Microscopy
Cell Biology
Eye Diseases
Hematoxylin
Eosine Yellowish-(YS)
Lipid Peroxidation
Reactive Oxygen Species
Enzyme-Linked Immunosorbent Assay
Prospective Studies
Membranes
4-hydroxy-2-nonenal

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

Evaluation of lipid oxidative stress status in Sjögren syndrome patients. / Wakamatsu, Tais H.; Dogru, Murat; Matsumoto, Yukihiro; Kojima, Takashi; Kaido, Minako; Ibrahim, Osama M A; Sato, Enrique A.; Igarashi, Ayako; Ichihashi, Yoshiyuki; Satake, Yoshiyuki; Shimazaki, Jun; Tsubota, Kazuo.

In: Investigative Ophthalmology and Visual Science, Vol. 54, No. 1, 01.2013, p. 201-210.

Research output: Contribution to journalArticle

Wakamatsu, TH, Dogru, M, Matsumoto, Y, Kojima, T, Kaido, M, Ibrahim, OMA, Sato, EA, Igarashi, A, Ichihashi, Y, Satake, Y, Shimazaki, J & Tsubota, K 2013, 'Evaluation of lipid oxidative stress status in Sjögren syndrome patients', Investigative Ophthalmology and Visual Science, vol. 54, no. 1, pp. 201-210. https://doi.org/10.1167/iovs.12-10325
Wakamatsu, Tais H. ; Dogru, Murat ; Matsumoto, Yukihiro ; Kojima, Takashi ; Kaido, Minako ; Ibrahim, Osama M A ; Sato, Enrique A. ; Igarashi, Ayako ; Ichihashi, Yoshiyuki ; Satake, Yoshiyuki ; Shimazaki, Jun ; Tsubota, Kazuo. / Evaluation of lipid oxidative stress status in Sjögren syndrome patients. In: Investigative Ophthalmology and Visual Science. 2013 ; Vol. 54, No. 1. pp. 201-210.
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AU - Ibrahim, Osama M A

AU - Sato, Enrique A.

AU - Igarashi, Ayako

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N2 - PURPOSE. We evaluated the levels of lipid oxidative stress markers and inflammatory cells from tears and conjunctiva of patients with Sjögren syndrome (SS) and normal subjects. METHODS. We examined 31 eyes of 16 patients (16 females) with SS and 15 eyes of 10 healthy controls (2 males and 8 females) in this prospective study. All subjects underwent a Schirmer test, measurement of tear film break-up time, vital stainings, confocal microscopy of the conjunctiva, tear collection for hexanoyl-lysine (HEL), ELISA, and conjunctival brush cytology. Brush cytology samples underwent immunohistochemistry (IHC) staining with HEL and 4-hydroxy-2- nonenal (4HNE). Hematoxylin-eosin and IHC staining with HEL and 4HNE also were performed on conjunctival samples of SS patients and controls. RESULTS. The tear stability and vital staining scores were significantly worse in eyes of SS patients compared to the controls. Conjunctival inflammatory cell density was significantly higher in SS subjects compared to controls. The numbers of conjunctival cells stained positively for HEL and 4HNE were significantly higher in SS patients compared to controls. Tear HEL concentrations correlated significantly with staining scores and inflammatory cell density in confocal microscopy. Conjunctival specimens also revealed higher numbers of cells stained positively for inflammatory markers, as well as HEL and 4HNE in the IHC stainings. CONCLUSIONS. Increase of the oxidative stress status in the conjunctiva of SS patients appears to have a role in the pathogenesis of dry eye disease. A close relationship may exist between reactive oxygen species (ROS) production, lipid peroxidation related membrane damage, and inflammatory processes in dry eye.

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