Evaluation of metabolic alteration in transgenic rice overexpressing dihydroflavonol-4-reductase

Hideyuki Takahashi, Mitsunori Hayashi, Fumiyuki Goto, Shigeru Sato, Tomoyoshi Soga, Takaaki Nishioka, Masaru Tomita, Maki Kawai-Yamada, Hirofumi Uchimiya

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Background and Aims: Previous studies have shown that transgenic rice plants overexpressing YK1, which possesses dihydroflavonol-4-reductase (DFR) activity, showed biotic and abiotic stress tolerance. High throughput profiles of metabolites have also been shown in such transgenic plants by Fourier transform ion cyclotron mass spectrometry. In this study, capillary electrophoresis mass spectrometry analysis (CE/MS) was employed to identify precise metabolites such as organic acids, amino acids and sugars. Methods: Using CE/MS, we analysed several metabolites of glycolysis, the tricarboxylic acid (TCA) cycle and the pentose phosphate pathway. In addition, the concentrations of sugars and ion were quantified. Key Results: In YK1 (DFR)-overexpressing plants, the concentrations of cis-aconitate, isocitrate and 2-oxoglutarate were higher in leaves, whereas those of fructose-1,6- bisphosphate and glyceraldehyde-3-phosphate were lower in roots. In seeds, the amounts of free amino acids and metals were altered, whereas sugars in seeds were kept constant. In YK1 calli, an approx. 3-fold increase in glutathione was observed, whereas the activities of glutathione peroxidase and glutathione reductase were concomitantly increased. Conclusions: The overexpression of YK1 (DFR) was associated with slight changes in the amounts of several metabolites analysed in whole plants, whilst glutathione derivatives were substantially increased in suspension-cultured cells.

Original languageEnglish
Pages (from-to)819-825
Number of pages7
JournalAnnals of Botany
Volume98
Issue number4
DOIs
Publication statusPublished - 2006 Oct

Fingerprint

genetically modified organisms
metabolites
rice
capillary electrophoresis
mass spectrometry
glutathione
glyceraldehyde 3-phosphate
ions
sugars
amino sugars
pentoses
tricarboxylic acid cycle
glycolysis
biotic stress
glutathione-disulfide reductase
seeds
glutathione peroxidase
stress tolerance
free amino acids
cultured cells

Keywords

  • Capillary electrophoresis
  • Dihydroflavonol-4-reductase
  • Mass spectrometry
  • Metabolome
  • Oryza sativa
  • Rice

ASJC Scopus subject areas

  • Horticulture
  • Plant Science

Cite this

Takahashi, H., Hayashi, M., Goto, F., Sato, S., Soga, T., Nishioka, T., ... Uchimiya, H. (2006). Evaluation of metabolic alteration in transgenic rice overexpressing dihydroflavonol-4-reductase. Annals of Botany, 98(4), 819-825. https://doi.org/10.1093/aob/mcl162

Evaluation of metabolic alteration in transgenic rice overexpressing dihydroflavonol-4-reductase. / Takahashi, Hideyuki; Hayashi, Mitsunori; Goto, Fumiyuki; Sato, Shigeru; Soga, Tomoyoshi; Nishioka, Takaaki; Tomita, Masaru; Kawai-Yamada, Maki; Uchimiya, Hirofumi.

In: Annals of Botany, Vol. 98, No. 4, 10.2006, p. 819-825.

Research output: Contribution to journalArticle

Takahashi, H, Hayashi, M, Goto, F, Sato, S, Soga, T, Nishioka, T, Tomita, M, Kawai-Yamada, M & Uchimiya, H 2006, 'Evaluation of metabolic alteration in transgenic rice overexpressing dihydroflavonol-4-reductase', Annals of Botany, vol. 98, no. 4, pp. 819-825. https://doi.org/10.1093/aob/mcl162
Takahashi, Hideyuki ; Hayashi, Mitsunori ; Goto, Fumiyuki ; Sato, Shigeru ; Soga, Tomoyoshi ; Nishioka, Takaaki ; Tomita, Masaru ; Kawai-Yamada, Maki ; Uchimiya, Hirofumi. / Evaluation of metabolic alteration in transgenic rice overexpressing dihydroflavonol-4-reductase. In: Annals of Botany. 2006 ; Vol. 98, No. 4. pp. 819-825.
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