Evaluation of phosphatidylserine-dependent antiprothrombin antibody testing for the diagnosis of antiphospholipid syndrome: Results of an international multicentre study

O. Amengual, R. Forastiero, M. Sugiura-Ogasawara, Kotaro Otomo, K. Oku, C. Favas, J. Delgado Alves, P. Žigon, A. Ambrožič, M. Tomšič, I. Ruiz-Arruza, G. Ruiz-Irastorza, M. L. Bertolaccini, G. L. Norman, Z. Shums, J. Arai, A. Murashima, A. E. Tebo, M. Gerosa, P. L. MeroniI. Rodriguez-Pintó, R. Cervera, J. Swadzba, J. Musial, T. Atsumi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Objective A task force of scientists at the International Congress on Antiphospholipid Antibodies recognized that phosphatidylserine-dependent antiprothrombin antibodies (aPS/PT) might contribute to a better identification of antiphospholipid syndrome (APS). Accordingly, initial and replication retrospective, cross-sectional multicentre studies were conducted to ascertain the value of aPS/PT for APS diagnosis. Methods In the initial study (eight centres, seven countries), clinical/laboratory data were retrospectively collected. Serum/plasma samples were tested for IgG aPS/PT at Inova Diagnostics (Inova) using two ELISA kits. A replication study (five centres, five countries) was carried out afterwards. Results In the initial study (n = 247), a moderate agreement between the IgG aPS/PT Inova and MBL ELISA kits was observed (k = 0.598). IgG aPS/PT were more prevalent in APS patients (51%) than in those without (9%), OR 10.8, 95% CI (4.0-29.3), p < 0.0001. Sensitivity, specificity, positive (LR+) and negative (LR-) likelihood ratio of IgG aPS/PT for APS diagnosis were 51%, 91%, 5.9 and 0.5, respectively. In the replication study (n = 214), a moderate/substantial agreement between the IgG aPS/PT results obtained with both ELISA kits was observed (k = 0.630). IgG aPS/PT were more prevalent in APS patients (47%) than in those without (12%), OR 6.4, 95% CI (2.6-16), p < 0.0001. Sensitivity, specificity, LR + and LR- for APS diagnosis were 47%, 88%, 3.9 and 0.6, respectively. Conclusions IgG aPS/PT detection is an easily performed laboratory parameter that might contribute to a better and more complete identification of patients with APS.

Original languageEnglish
Pages (from-to)266-276
Number of pages11
JournalLupus
Volume26
Issue number3
DOIs
Publication statusPublished - 2017 Jan 1
Externally publishedYes

Fingerprint

Antiphospholipid Syndrome
Phosphatidylserines
Multicenter Studies
Immunoglobulin G
Antibodies
Enzyme-Linked Immunosorbent Assay
Sensitivity and Specificity
Antiphospholipid Antibodies
Advisory Committees
Cross-Sectional Studies
Serum

Keywords

  • Antiphospholipid antibodies
  • lupus anticoagulant
  • systemic lupus erythematosus
  • thrombosis

ASJC Scopus subject areas

  • Rheumatology

Cite this

Evaluation of phosphatidylserine-dependent antiprothrombin antibody testing for the diagnosis of antiphospholipid syndrome : Results of an international multicentre study. / Amengual, O.; Forastiero, R.; Sugiura-Ogasawara, M.; Otomo, Kotaro; Oku, K.; Favas, C.; Delgado Alves, J.; Žigon, P.; Ambrožič, A.; Tomšič, M.; Ruiz-Arruza, I.; Ruiz-Irastorza, G.; Bertolaccini, M. L.; Norman, G. L.; Shums, Z.; Arai, J.; Murashima, A.; Tebo, A. E.; Gerosa, M.; Meroni, P. L.; Rodriguez-Pintó, I.; Cervera, R.; Swadzba, J.; Musial, J.; Atsumi, T.

In: Lupus, Vol. 26, No. 3, 01.01.2017, p. 266-276.

Research output: Contribution to journalArticle

Amengual, O, Forastiero, R, Sugiura-Ogasawara, M, Otomo, K, Oku, K, Favas, C, Delgado Alves, J, Žigon, P, Ambrožič, A, Tomšič, M, Ruiz-Arruza, I, Ruiz-Irastorza, G, Bertolaccini, ML, Norman, GL, Shums, Z, Arai, J, Murashima, A, Tebo, AE, Gerosa, M, Meroni, PL, Rodriguez-Pintó, I, Cervera, R, Swadzba, J, Musial, J & Atsumi, T 2017, 'Evaluation of phosphatidylserine-dependent antiprothrombin antibody testing for the diagnosis of antiphospholipid syndrome: Results of an international multicentre study', Lupus, vol. 26, no. 3, pp. 266-276. https://doi.org/10.1177/0961203316660203
Amengual, O. ; Forastiero, R. ; Sugiura-Ogasawara, M. ; Otomo, Kotaro ; Oku, K. ; Favas, C. ; Delgado Alves, J. ; Žigon, P. ; Ambrožič, A. ; Tomšič, M. ; Ruiz-Arruza, I. ; Ruiz-Irastorza, G. ; Bertolaccini, M. L. ; Norman, G. L. ; Shums, Z. ; Arai, J. ; Murashima, A. ; Tebo, A. E. ; Gerosa, M. ; Meroni, P. L. ; Rodriguez-Pintó, I. ; Cervera, R. ; Swadzba, J. ; Musial, J. ; Atsumi, T. / Evaluation of phosphatidylserine-dependent antiprothrombin antibody testing for the diagnosis of antiphospholipid syndrome : Results of an international multicentre study. In: Lupus. 2017 ; Vol. 26, No. 3. pp. 266-276.
@article{d302379eddd8420b95984ede9a13b616,
title = "Evaluation of phosphatidylserine-dependent antiprothrombin antibody testing for the diagnosis of antiphospholipid syndrome: Results of an international multicentre study",
abstract = "Objective A task force of scientists at the International Congress on Antiphospholipid Antibodies recognized that phosphatidylserine-dependent antiprothrombin antibodies (aPS/PT) might contribute to a better identification of antiphospholipid syndrome (APS). Accordingly, initial and replication retrospective, cross-sectional multicentre studies were conducted to ascertain the value of aPS/PT for APS diagnosis. Methods In the initial study (eight centres, seven countries), clinical/laboratory data were retrospectively collected. Serum/plasma samples were tested for IgG aPS/PT at Inova Diagnostics (Inova) using two ELISA kits. A replication study (five centres, five countries) was carried out afterwards. Results In the initial study (n = 247), a moderate agreement between the IgG aPS/PT Inova and MBL ELISA kits was observed (k = 0.598). IgG aPS/PT were more prevalent in APS patients (51{\%}) than in those without (9{\%}), OR 10.8, 95{\%} CI (4.0-29.3), p < 0.0001. Sensitivity, specificity, positive (LR+) and negative (LR-) likelihood ratio of IgG aPS/PT for APS diagnosis were 51{\%}, 91{\%}, 5.9 and 0.5, respectively. In the replication study (n = 214), a moderate/substantial agreement between the IgG aPS/PT results obtained with both ELISA kits was observed (k = 0.630). IgG aPS/PT were more prevalent in APS patients (47{\%}) than in those without (12{\%}), OR 6.4, 95{\%} CI (2.6-16), p < 0.0001. Sensitivity, specificity, LR + and LR- for APS diagnosis were 47{\%}, 88{\%}, 3.9 and 0.6, respectively. Conclusions IgG aPS/PT detection is an easily performed laboratory parameter that might contribute to a better and more complete identification of patients with APS.",
keywords = "Antiphospholipid antibodies, lupus anticoagulant, systemic lupus erythematosus, thrombosis",
author = "O. Amengual and R. Forastiero and M. Sugiura-Ogasawara and Kotaro Otomo and K. Oku and C. Favas and {Delgado Alves}, J. and P. Žigon and A. Ambrožič and M. Tomšič and I. Ruiz-Arruza and G. Ruiz-Irastorza and Bertolaccini, {M. L.} and Norman, {G. L.} and Z. Shums and J. Arai and A. Murashima and Tebo, {A. E.} and M. Gerosa and Meroni, {P. L.} and I. Rodriguez-Pint{\'o} and R. Cervera and J. Swadzba and J. Musial and T. Atsumi",
year = "2017",
month = "1",
day = "1",
doi = "10.1177/0961203316660203",
language = "English",
volume = "26",
pages = "266--276",
journal = "Lupus",
issn = "0961-2033",
publisher = "SAGE Publications Ltd",
number = "3",

}

TY - JOUR

T1 - Evaluation of phosphatidylserine-dependent antiprothrombin antibody testing for the diagnosis of antiphospholipid syndrome

T2 - Results of an international multicentre study

AU - Amengual, O.

AU - Forastiero, R.

AU - Sugiura-Ogasawara, M.

AU - Otomo, Kotaro

AU - Oku, K.

AU - Favas, C.

AU - Delgado Alves, J.

AU - Žigon, P.

AU - Ambrožič, A.

AU - Tomšič, M.

AU - Ruiz-Arruza, I.

AU - Ruiz-Irastorza, G.

AU - Bertolaccini, M. L.

AU - Norman, G. L.

AU - Shums, Z.

AU - Arai, J.

AU - Murashima, A.

AU - Tebo, A. E.

AU - Gerosa, M.

AU - Meroni, P. L.

AU - Rodriguez-Pintó, I.

AU - Cervera, R.

AU - Swadzba, J.

AU - Musial, J.

AU - Atsumi, T.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Objective A task force of scientists at the International Congress on Antiphospholipid Antibodies recognized that phosphatidylserine-dependent antiprothrombin antibodies (aPS/PT) might contribute to a better identification of antiphospholipid syndrome (APS). Accordingly, initial and replication retrospective, cross-sectional multicentre studies were conducted to ascertain the value of aPS/PT for APS diagnosis. Methods In the initial study (eight centres, seven countries), clinical/laboratory data were retrospectively collected. Serum/plasma samples were tested for IgG aPS/PT at Inova Diagnostics (Inova) using two ELISA kits. A replication study (five centres, five countries) was carried out afterwards. Results In the initial study (n = 247), a moderate agreement between the IgG aPS/PT Inova and MBL ELISA kits was observed (k = 0.598). IgG aPS/PT were more prevalent in APS patients (51%) than in those without (9%), OR 10.8, 95% CI (4.0-29.3), p < 0.0001. Sensitivity, specificity, positive (LR+) and negative (LR-) likelihood ratio of IgG aPS/PT for APS diagnosis were 51%, 91%, 5.9 and 0.5, respectively. In the replication study (n = 214), a moderate/substantial agreement between the IgG aPS/PT results obtained with both ELISA kits was observed (k = 0.630). IgG aPS/PT were more prevalent in APS patients (47%) than in those without (12%), OR 6.4, 95% CI (2.6-16), p < 0.0001. Sensitivity, specificity, LR + and LR- for APS diagnosis were 47%, 88%, 3.9 and 0.6, respectively. Conclusions IgG aPS/PT detection is an easily performed laboratory parameter that might contribute to a better and more complete identification of patients with APS.

AB - Objective A task force of scientists at the International Congress on Antiphospholipid Antibodies recognized that phosphatidylserine-dependent antiprothrombin antibodies (aPS/PT) might contribute to a better identification of antiphospholipid syndrome (APS). Accordingly, initial and replication retrospective, cross-sectional multicentre studies were conducted to ascertain the value of aPS/PT for APS diagnosis. Methods In the initial study (eight centres, seven countries), clinical/laboratory data were retrospectively collected. Serum/plasma samples were tested for IgG aPS/PT at Inova Diagnostics (Inova) using two ELISA kits. A replication study (five centres, five countries) was carried out afterwards. Results In the initial study (n = 247), a moderate agreement between the IgG aPS/PT Inova and MBL ELISA kits was observed (k = 0.598). IgG aPS/PT were more prevalent in APS patients (51%) than in those without (9%), OR 10.8, 95% CI (4.0-29.3), p < 0.0001. Sensitivity, specificity, positive (LR+) and negative (LR-) likelihood ratio of IgG aPS/PT for APS diagnosis were 51%, 91%, 5.9 and 0.5, respectively. In the replication study (n = 214), a moderate/substantial agreement between the IgG aPS/PT results obtained with both ELISA kits was observed (k = 0.630). IgG aPS/PT were more prevalent in APS patients (47%) than in those without (12%), OR 6.4, 95% CI (2.6-16), p < 0.0001. Sensitivity, specificity, LR + and LR- for APS diagnosis were 47%, 88%, 3.9 and 0.6, respectively. Conclusions IgG aPS/PT detection is an easily performed laboratory parameter that might contribute to a better and more complete identification of patients with APS.

KW - Antiphospholipid antibodies

KW - lupus anticoagulant

KW - systemic lupus erythematosus

KW - thrombosis

UR - http://www.scopus.com/inward/record.url?scp=85011547195&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85011547195&partnerID=8YFLogxK

U2 - 10.1177/0961203316660203

DO - 10.1177/0961203316660203

M3 - Article

C2 - 27510605

AN - SCOPUS:85011547195

VL - 26

SP - 266

EP - 276

JO - Lupus

JF - Lupus

SN - 0961-2033

IS - 3

ER -