Evaluation of Sycp3, Plzf and cyclin B3 expression and suitability as spermatogonia and spermatocyte markers in zebrafish

Yuichi Ozaki, Kenji Saito, Minori Shinya, Toshihiro Kawasaki, Noriyoshi Sakai

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Recent studies in mammals have revealed the heterogeneity of spermatogonial populations which contain differentiated and undifferentiated cells that further divide into actual stem cells and potential stem cells. In fish however, there are no functional definitions, and very few molecular markers, for germ cells. In our present study, specific antibodies were raised against Sycp3, Plzf and Cyclin B3 in zebrafish and then used to determine the localization of these proteins in the testis. We wished to confirm whether these molecules were potential markers for spermatocytes and spermatogonia. Immunohistochemical observations revealed that Sycp3 is specifically localized in spermatocytes in typical nuclear patterns at each meiotic stage. Plzf was found to be localized in the nucleus of both type A and type B spermatogonia until the 8-cell clone, similar to the pattern in Plzf-positive Asingle-Aaligned undifferentiated spermatogonia in rodents. In addition to Plzf, the localization of Cyclin B3 was predominantly detected in the nuclei of type A and early type B spermatogonia until the 16-cell clone. Additionally, Cyclin B3 protein signals were detected in germ cells in large cysts, possibly corresponding to spermatocytes at the preleptotene stage. Our present data thus show that these molecules have properties that will enable their use as markers of spermatocytes and early spermatogonia in zebrafish.

Original languageEnglish
Pages (from-to)309-315
Number of pages7
JournalGene Expression Patterns
Volume11
Issue number5-6
DOIs
Publication statusPublished - 2011 Jun
Externally publishedYes

Fingerprint

Spermatogonia
Cyclins
Spermatocytes
Zebrafish
Germ Cells
Stem Cells
Clone Cells
Population Characteristics
Testis
Cysts
Mammals
Rodentia
Fishes
Proteins
Antibodies

Keywords

  • Cyclin B3
  • Plzf
  • Spermatocyte
  • Spermatogonia
  • Sycp3
  • Testis
  • Zebrafish

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Developmental Biology

Cite this

Evaluation of Sycp3, Plzf and cyclin B3 expression and suitability as spermatogonia and spermatocyte markers in zebrafish. / Ozaki, Yuichi; Saito, Kenji; Shinya, Minori; Kawasaki, Toshihiro; Sakai, Noriyoshi.

In: Gene Expression Patterns, Vol. 11, No. 5-6, 06.2011, p. 309-315.

Research output: Contribution to journalArticle

Ozaki, Yuichi ; Saito, Kenji ; Shinya, Minori ; Kawasaki, Toshihiro ; Sakai, Noriyoshi. / Evaluation of Sycp3, Plzf and cyclin B3 expression and suitability as spermatogonia and spermatocyte markers in zebrafish. In: Gene Expression Patterns. 2011 ; Vol. 11, No. 5-6. pp. 309-315.
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