Evaluation of the rapid immunochromatographic ODK0501 assay for Streptococcus pneumoniae antigen detection with nasopharyngeal swabs: Preliminary report

Shoji Suzuki, Naoki Nishimura, Torahiko Jinta, Yasuhiko Yamano, Genta Ishikawa, Yutaka Tomishima, Noboru Uchiyama, Naohiko Chohnabayashi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Early identification and control of pathogenic bacteria are important in the treatment of pneumonia. Currently, two rapid antigen detection kits for pneumococcal pneumonia are available: one uses urine samples and the other, named RAPIRUN® S. pneumoniae, uses sputum samples. RAPIRUN® has shown high sensitivity with nasopharyngeal swab samples from pediatric patients. In this study, we investigated the performance of RAPIRUN® with nasopharyngeal swabs from adult patients. Methods: All adult patients diagnosed with pneumonia from November 2011 to April 2012 in St. Luke's International hospital were included in this cross-sectional study. Single sputum, nasopharyngeal swab, and urine samples obtained from patients were investigated using a rapid antigen detection kit. Sputum and blood cultures were also evaluated. We compared the characteristics of pneumococcal pneumonia patients diagnosed using RAPIRUN with a nasopharyngeal swab to those patients diagnosed using other methods. Sensitivity and specificity were also calculated. Results: Seventeen out of 60 patients with pneumonia were diagnosed with pneumococcal pneumonia. In 4 out of the 17 cases, a positive test result was obtained using RAPIRUN with a nasopharyngeal swab. The sensitivity and specificity were 23.5 and 100 %, respectively. Conclusion: RAPIRUN performed with nasopharyngeal swabs from adult patients exhibited lower sensitivity for the diagnosis of pneumococcal pneumonia than the other compared methods. The causative pathogen of pneumonia should be identified using not only sputum cultures or rapid antigen detection kits but also clinical features or gram staining of sputum.

Original languageEnglish
Article number25
JournalMultidisciplinary Respiratory Medicine
Volume11
Issue number1
DOIs
Publication statusPublished - 2016 Jun 20
Externally publishedYes

Fingerprint

Immunochromatography
Streptococcus pneumoniae
Pneumococcal Pneumonia
Antigens
Sputum
Pneumonia
Urine
Sensitivity and Specificity
Cross-Sectional Studies
Pediatrics
Staining and Labeling
Bacteria

Keywords

  • Immunochromatographic assay
  • Nasopharyngeal swab
  • Pneumonia
  • RAPIRUN® S. pneumoniae

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Evaluation of the rapid immunochromatographic ODK0501 assay for Streptococcus pneumoniae antigen detection with nasopharyngeal swabs : Preliminary report. / Suzuki, Shoji; Nishimura, Naoki; Jinta, Torahiko; Yamano, Yasuhiko; Ishikawa, Genta; Tomishima, Yutaka; Uchiyama, Noboru; Chohnabayashi, Naohiko.

In: Multidisciplinary Respiratory Medicine, Vol. 11, No. 1, 25, 20.06.2016.

Research output: Contribution to journalArticle

Suzuki, Shoji ; Nishimura, Naoki ; Jinta, Torahiko ; Yamano, Yasuhiko ; Ishikawa, Genta ; Tomishima, Yutaka ; Uchiyama, Noboru ; Chohnabayashi, Naohiko. / Evaluation of the rapid immunochromatographic ODK0501 assay for Streptococcus pneumoniae antigen detection with nasopharyngeal swabs : Preliminary report. In: Multidisciplinary Respiratory Medicine. 2016 ; Vol. 11, No. 1.
@article{6f7e888091454fa695239688276d6f3d,
title = "Evaluation of the rapid immunochromatographic ODK0501 assay for Streptococcus pneumoniae antigen detection with nasopharyngeal swabs: Preliminary report",
abstract = "Background: Early identification and control of pathogenic bacteria are important in the treatment of pneumonia. Currently, two rapid antigen detection kits for pneumococcal pneumonia are available: one uses urine samples and the other, named RAPIRUN{\circledR} S. pneumoniae, uses sputum samples. RAPIRUN{\circledR} has shown high sensitivity with nasopharyngeal swab samples from pediatric patients. In this study, we investigated the performance of RAPIRUN{\circledR} with nasopharyngeal swabs from adult patients. Methods: All adult patients diagnosed with pneumonia from November 2011 to April 2012 in St. Luke's International hospital were included in this cross-sectional study. Single sputum, nasopharyngeal swab, and urine samples obtained from patients were investigated using a rapid antigen detection kit. Sputum and blood cultures were also evaluated. We compared the characteristics of pneumococcal pneumonia patients diagnosed using RAPIRUN with a nasopharyngeal swab to those patients diagnosed using other methods. Sensitivity and specificity were also calculated. Results: Seventeen out of 60 patients with pneumonia were diagnosed with pneumococcal pneumonia. In 4 out of the 17 cases, a positive test result was obtained using RAPIRUN with a nasopharyngeal swab. The sensitivity and specificity were 23.5 and 100 {\%}, respectively. Conclusion: RAPIRUN performed with nasopharyngeal swabs from adult patients exhibited lower sensitivity for the diagnosis of pneumococcal pneumonia than the other compared methods. The causative pathogen of pneumonia should be identified using not only sputum cultures or rapid antigen detection kits but also clinical features or gram staining of sputum.",
keywords = "Immunochromatographic assay, Nasopharyngeal swab, Pneumonia, RAPIRUN{\circledR} S. pneumoniae",
author = "Shoji Suzuki and Naoki Nishimura and Torahiko Jinta and Yasuhiko Yamano and Genta Ishikawa and Yutaka Tomishima and Noboru Uchiyama and Naohiko Chohnabayashi",
year = "2016",
month = "6",
day = "20",
doi = "10.1186/s40248-016-0060-5",
language = "English",
volume = "11",
journal = "Multidisciplinary Respiratory Medicine",
issn = "1828-695X",
publisher = "Novamedia s.r.l.",
number = "1",

}

TY - JOUR

T1 - Evaluation of the rapid immunochromatographic ODK0501 assay for Streptococcus pneumoniae antigen detection with nasopharyngeal swabs

T2 - Preliminary report

AU - Suzuki, Shoji

AU - Nishimura, Naoki

AU - Jinta, Torahiko

AU - Yamano, Yasuhiko

AU - Ishikawa, Genta

AU - Tomishima, Yutaka

AU - Uchiyama, Noboru

AU - Chohnabayashi, Naohiko

PY - 2016/6/20

Y1 - 2016/6/20

N2 - Background: Early identification and control of pathogenic bacteria are important in the treatment of pneumonia. Currently, two rapid antigen detection kits for pneumococcal pneumonia are available: one uses urine samples and the other, named RAPIRUN® S. pneumoniae, uses sputum samples. RAPIRUN® has shown high sensitivity with nasopharyngeal swab samples from pediatric patients. In this study, we investigated the performance of RAPIRUN® with nasopharyngeal swabs from adult patients. Methods: All adult patients diagnosed with pneumonia from November 2011 to April 2012 in St. Luke's International hospital were included in this cross-sectional study. Single sputum, nasopharyngeal swab, and urine samples obtained from patients were investigated using a rapid antigen detection kit. Sputum and blood cultures were also evaluated. We compared the characteristics of pneumococcal pneumonia patients diagnosed using RAPIRUN with a nasopharyngeal swab to those patients diagnosed using other methods. Sensitivity and specificity were also calculated. Results: Seventeen out of 60 patients with pneumonia were diagnosed with pneumococcal pneumonia. In 4 out of the 17 cases, a positive test result was obtained using RAPIRUN with a nasopharyngeal swab. The sensitivity and specificity were 23.5 and 100 %, respectively. Conclusion: RAPIRUN performed with nasopharyngeal swabs from adult patients exhibited lower sensitivity for the diagnosis of pneumococcal pneumonia than the other compared methods. The causative pathogen of pneumonia should be identified using not only sputum cultures or rapid antigen detection kits but also clinical features or gram staining of sputum.

AB - Background: Early identification and control of pathogenic bacteria are important in the treatment of pneumonia. Currently, two rapid antigen detection kits for pneumococcal pneumonia are available: one uses urine samples and the other, named RAPIRUN® S. pneumoniae, uses sputum samples. RAPIRUN® has shown high sensitivity with nasopharyngeal swab samples from pediatric patients. In this study, we investigated the performance of RAPIRUN® with nasopharyngeal swabs from adult patients. Methods: All adult patients diagnosed with pneumonia from November 2011 to April 2012 in St. Luke's International hospital were included in this cross-sectional study. Single sputum, nasopharyngeal swab, and urine samples obtained from patients were investigated using a rapid antigen detection kit. Sputum and blood cultures were also evaluated. We compared the characteristics of pneumococcal pneumonia patients diagnosed using RAPIRUN with a nasopharyngeal swab to those patients diagnosed using other methods. Sensitivity and specificity were also calculated. Results: Seventeen out of 60 patients with pneumonia were diagnosed with pneumococcal pneumonia. In 4 out of the 17 cases, a positive test result was obtained using RAPIRUN with a nasopharyngeal swab. The sensitivity and specificity were 23.5 and 100 %, respectively. Conclusion: RAPIRUN performed with nasopharyngeal swabs from adult patients exhibited lower sensitivity for the diagnosis of pneumococcal pneumonia than the other compared methods. The causative pathogen of pneumonia should be identified using not only sputum cultures or rapid antigen detection kits but also clinical features or gram staining of sputum.

KW - Immunochromatographic assay

KW - Nasopharyngeal swab

KW - Pneumonia

KW - RAPIRUN® S. pneumoniae

UR - http://www.scopus.com/inward/record.url?scp=84975874039&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84975874039&partnerID=8YFLogxK

U2 - 10.1186/s40248-016-0060-5

DO - 10.1186/s40248-016-0060-5

M3 - Article

AN - SCOPUS:84975874039

VL - 11

JO - Multidisciplinary Respiratory Medicine

JF - Multidisciplinary Respiratory Medicine

SN - 1828-695X

IS - 1

M1 - 25

ER -