Evaluation of the subacute pulmonary and testicular inhalation toxicity of diborane in rats

Tetsuo Nomiyama, Kazuyuki Omae, Chizuru Ishizuka, Kanae Hosoda, Yuko Yamano, Hiroshi Nakashima, Takamoto Uemura, Haruhiko Sakurai

Research output: Contribution to journalArticle

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Abstract

This study aimed to clarify the subacute pulmonary and testicular inhalation toxicity of diborane (B2H6, CAS: 19287-45-7) in rats. Male Wistar rats were exposed for 8 weeks to 0.11 or 0.96 ppm of diborane for 6 hr/day, 5 days/week. The control group was exposed to filtered air. Bronchoalveolar lavage fluid (BALE), hematological, biochemical, and histopathological examinations were conducted. Sperm counts and spermatic morphological changes were examined in epididymides, and histopathological examination was carried out in testes. BALF examinations revealed that the percentage of neutrophils increased in a dose-dependent manner and that of macrophages decreased in rats exposed to 0.96 ppm. Quantities of total and individual phospholipids in BALF increased in rats exposed to 0.96 ppm. The proportion of phosphatidylglycerol plus sphingomyelin decreased, and phosphatidylethanolamine and phosphatidylinositol increased in rats exposed to 0.96 ppm. LDH increased in rats exposed to 0.96 ppm, and ALP showed a dose-dependent increase. In serum, α1-antitrypsin and superoxide dismutase activities increased in rats exposed to 0.11 or 0.96 ppm. These changes showed dose-dependent effects on the lung in rats exposed to diborane, possibly indicating that the hyperenergia of type II cells with proliferation and/or hypertrophy without histopathological changes occurred even in rats exposed to 0.11 ppm. Testicular examinations revealed no particular findings. The TLV-TWA of diborane (0.1 ppm) seems to be high and possibly unsafe, considering that the no-observed-effect level over 8 weeks for rat lung was under 0.11 ppm.

Original languageEnglish
Pages (from-to)77-83
Number of pages7
JournalToxicology and Applied Pharmacology
Volume138
Issue number1
DOIs
Publication statusPublished - 1996 May

Fingerprint

Inhalation
Toxicity
Rats
Lung
Threshold Limit Values
diborane
No-Observed-Adverse-Effect Level
Phosphatidylglycerols
Sperm Count
Sphingomyelins
Epididymis
Bronchoalveolar Lavage Fluid
Macrophages
Phosphatidylinositols
Hypertrophy
Superoxide Dismutase
Spermatozoa
Testis
Wistar Rats
Phospholipids

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Nomiyama, T., Omae, K., Ishizuka, C., Hosoda, K., Yamano, Y., Nakashima, H., ... Sakurai, H. (1996). Evaluation of the subacute pulmonary and testicular inhalation toxicity of diborane in rats. Toxicology and Applied Pharmacology, 138(1), 77-83. https://doi.org/10.1006/taap.1996.0100

Evaluation of the subacute pulmonary and testicular inhalation toxicity of diborane in rats. / Nomiyama, Tetsuo; Omae, Kazuyuki; Ishizuka, Chizuru; Hosoda, Kanae; Yamano, Yuko; Nakashima, Hiroshi; Uemura, Takamoto; Sakurai, Haruhiko.

In: Toxicology and Applied Pharmacology, Vol. 138, No. 1, 05.1996, p. 77-83.

Research output: Contribution to journalArticle

Nomiyama, T, Omae, K, Ishizuka, C, Hosoda, K, Yamano, Y, Nakashima, H, Uemura, T & Sakurai, H 1996, 'Evaluation of the subacute pulmonary and testicular inhalation toxicity of diborane in rats', Toxicology and Applied Pharmacology, vol. 138, no. 1, pp. 77-83. https://doi.org/10.1006/taap.1996.0100
Nomiyama, Tetsuo ; Omae, Kazuyuki ; Ishizuka, Chizuru ; Hosoda, Kanae ; Yamano, Yuko ; Nakashima, Hiroshi ; Uemura, Takamoto ; Sakurai, Haruhiko. / Evaluation of the subacute pulmonary and testicular inhalation toxicity of diborane in rats. In: Toxicology and Applied Pharmacology. 1996 ; Vol. 138, No. 1. pp. 77-83.
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