Evidence for active acetylcholine metabolism in human amniotic epithelial cells: Applicable to intracerebral allografting for neurologic disease

Norio Sakuragawa, Hidemi Misawa, Keiko Ohsugi, Kouji Kakishita, Takashi Ishii, Ramasamy Thangavel, Jun Tohyama, Mohamed Elwan, Yasunobu Yokoyama, Osamu Okuda, Hajime Arai, Ikuko Ogino, Kiyoshi Sato

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Abstract

Human amniotic epithelial (HAE) cells have been used for allotransplantation in patients with lysosomal storage disease due to lack of expression of HLA antigens. Previously, we have reported the expression of differentiation markers for both neural stem cells, and neuron and glial cells. In the present study, we investigated the presence of choline acetyltransferase (CHAT) and acetylcholine (ACh) in HAE cells using different experimental approaches. Cultured HAE cells showed strong immunoreactivity against ChAT antibody. ChAT activity in primary cells was 24.9 ±8.5 pmol/mg protein/h. Using HPLC with electrochemical detection, ACh was detected in both cell incubation media and cell pellets indicating that these cells synthesize and release ACh in a time-dependent manner. Additional confirmation of this hypothesis was gained from the data obtained from RT- PCR and Western blot analyses which revealed the expression of ChAT mRNA and ChAT protein, respectively, in HAE cells. Results of the present study suggest that HAE cells can possibly be applied for intracerebral allografting to treat neurologic diseases in which cholinergic neurons are damaged.

Original languageEnglish
Pages (from-to)53-56
Number of pages4
JournalNeuroscience Letters
Volume232
Issue number1
DOIs
Publication statusPublished - 1997 Aug 22

Keywords

  • Acetylcholine
  • Choline acetyltransferase
  • Human amniotic epithelial cells

ASJC Scopus subject areas

  • Neuroscience(all)

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    Sakuragawa, N., Misawa, H., Ohsugi, K., Kakishita, K., Ishii, T., Thangavel, R., Tohyama, J., Elwan, M., Yokoyama, Y., Okuda, O., Arai, H., Ogino, I., & Sato, K. (1997). Evidence for active acetylcholine metabolism in human amniotic epithelial cells: Applicable to intracerebral allografting for neurologic disease. Neuroscience Letters, 232(1), 53-56. https://doi.org/10.1016/S0304-3940(97)00570-3