Evidence for chloramphenicol/H+ antiport in cmr (MdfA) system of Escherichia coli and properties of the antiporter

Tomoyuki Mine, Yuji Morita, Atsuko Kataoka, Tohru Mizushima, Tomofusa Tsuchiya

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44 Citations (Scopus)

Abstract

We detected chloramphenicol/H+ antiport activity in membrane vesicles of Escherichia coli and cloned a gene for the antiporter from chromosomal DNA of E. coli. Introduction of the gene into E. coli cells conferred resistance to chloramphenicol and ethidium. A slight increase in resistance to acridine orange was also observed. Elevated chloramphenicol efflux and ethidium efflux were observed in cells harboring a plasmid carrying the gene. Addition of chloramphenicol to the assay mixture reduced the efflux of ethidium. Elevated chloramphenicol/H+ antiport activity was observed in membrane vesicles prepared from cells harboring the plasmid. The pH optimum for the activity was 6.5. We sequenced the gene and deduced the amino acid sequence of its product. A sequence homology search revealed that it was same as that of Cmr (or MdfA). Thus, it became clear that Cmr (MdfA) is the chloramphenicol(and ethidium)/H+ antiporter.

Original languageEnglish
Pages (from-to)187-193
Number of pages7
JournalJournal of biochemistry
Volume124
Issue number1
DOIs
Publication statusPublished - 1998 Jul

Keywords

  • Chloramphenicol/H
  • E. coli
  • Multidrug resistance
  • Sequence

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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