Evidence for creatine biosynthesis in Müller glia

Toshihisa Nakashima, Masatoshi Tomi, Masanori Tachikawa, Masahiko Watanabe, Tetsuya Terasaki, Ken Ichi Hosoya

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

In high-energy metabolic tissues like the retina, creatine may play an important role in energy storage and in transmission of phosphate-bound energy substrates. To prove this, we investigated creatine synthesis in Müller glia. We also characterized the localization of the creatine synthetic enzyme, S-adenosyl-L-methionine:N-guanidinoacetate methyltransferase (GAMT) in the retina. Reverse transcription-polymerase chain reaction analysis revealed that L-arginine:glycine amidinotransferase and GAMT mRNAs were expressed in the retina and the Müller cell line, TR-MUL5. [14C]Creatine was detected after incubation of isolated rat retina or TR-MUL5 cells with [ 14C]glycine, L-arginine and L-methionine, suggesting creatine synthesis in Müller glia. Western blot analysis also revealed expression of GAMT protein in the rat retina and TR-MUL5 cells. Furthermore, confocal immunofluorescent microscopy of dual-labeled rat retinal sections demonstrated co-localization of GAMT with glutamine synthetase. Taken together, the results of the present study indicate creatine synthesis in Müller glia, implying an important role of creatine in energy metabolism in the retina.

Original languageEnglish
Pages (from-to)47-52
Number of pages6
JournalGLIA
Volume52
Issue number1
DOIs
Publication statusPublished - 2005 Oct
Externally publishedYes

Fingerprint

Creatine
Neuroglia
Retina
Guanidinoacetate N-Methyltransferase
S-Adenosylmethionine
Glutamate-Ammonia Ligase
Confocal Microscopy
Methionine
Glycine
Energy Metabolism
Reverse Transcription
Arginine
Western Blotting
Phosphates
Cell Line
Polymerase Chain Reaction
Messenger RNA
Enzymes

Keywords

  • GAMT
  • Glutamine synthetase
  • Immunohistochemistry
  • Müller cell line
  • Rat retina

ASJC Scopus subject areas

  • Immunology

Cite this

Nakashima, T., Tomi, M., Tachikawa, M., Watanabe, M., Terasaki, T., & Hosoya, K. I. (2005). Evidence for creatine biosynthesis in Müller glia. GLIA, 52(1), 47-52. https://doi.org/10.1002/glia.20222

Evidence for creatine biosynthesis in Müller glia. / Nakashima, Toshihisa; Tomi, Masatoshi; Tachikawa, Masanori; Watanabe, Masahiko; Terasaki, Tetsuya; Hosoya, Ken Ichi.

In: GLIA, Vol. 52, No. 1, 10.2005, p. 47-52.

Research output: Contribution to journalArticle

Nakashima, T, Tomi, M, Tachikawa, M, Watanabe, M, Terasaki, T & Hosoya, KI 2005, 'Evidence for creatine biosynthesis in Müller glia', GLIA, vol. 52, no. 1, pp. 47-52. https://doi.org/10.1002/glia.20222
Nakashima T, Tomi M, Tachikawa M, Watanabe M, Terasaki T, Hosoya KI. Evidence for creatine biosynthesis in Müller glia. GLIA. 2005 Oct;52(1):47-52. https://doi.org/10.1002/glia.20222
Nakashima, Toshihisa ; Tomi, Masatoshi ; Tachikawa, Masanori ; Watanabe, Masahiko ; Terasaki, Tetsuya ; Hosoya, Ken Ichi. / Evidence for creatine biosynthesis in Müller glia. In: GLIA. 2005 ; Vol. 52, No. 1. pp. 47-52.
@article{3f19ecd5b1434911b05a3ea3ad0f8472,
title = "Evidence for creatine biosynthesis in M{\"u}ller glia",
abstract = "In high-energy metabolic tissues like the retina, creatine may play an important role in energy storage and in transmission of phosphate-bound energy substrates. To prove this, we investigated creatine synthesis in M{\"u}ller glia. We also characterized the localization of the creatine synthetic enzyme, S-adenosyl-L-methionine:N-guanidinoacetate methyltransferase (GAMT) in the retina. Reverse transcription-polymerase chain reaction analysis revealed that L-arginine:glycine amidinotransferase and GAMT mRNAs were expressed in the retina and the M{\"u}ller cell line, TR-MUL5. [14C]Creatine was detected after incubation of isolated rat retina or TR-MUL5 cells with [ 14C]glycine, L-arginine and L-methionine, suggesting creatine synthesis in M{\"u}ller glia. Western blot analysis also revealed expression of GAMT protein in the rat retina and TR-MUL5 cells. Furthermore, confocal immunofluorescent microscopy of dual-labeled rat retinal sections demonstrated co-localization of GAMT with glutamine synthetase. Taken together, the results of the present study indicate creatine synthesis in M{\"u}ller glia, implying an important role of creatine in energy metabolism in the retina.",
keywords = "GAMT, Glutamine synthetase, Immunohistochemistry, M{\"u}ller cell line, Rat retina",
author = "Toshihisa Nakashima and Masatoshi Tomi and Masanori Tachikawa and Masahiko Watanabe and Tetsuya Terasaki and Hosoya, {Ken Ichi}",
year = "2005",
month = "10",
doi = "10.1002/glia.20222",
language = "English",
volume = "52",
pages = "47--52",
journal = "GLIA",
issn = "0894-1491",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - Evidence for creatine biosynthesis in Müller glia

AU - Nakashima, Toshihisa

AU - Tomi, Masatoshi

AU - Tachikawa, Masanori

AU - Watanabe, Masahiko

AU - Terasaki, Tetsuya

AU - Hosoya, Ken Ichi

PY - 2005/10

Y1 - 2005/10

N2 - In high-energy metabolic tissues like the retina, creatine may play an important role in energy storage and in transmission of phosphate-bound energy substrates. To prove this, we investigated creatine synthesis in Müller glia. We also characterized the localization of the creatine synthetic enzyme, S-adenosyl-L-methionine:N-guanidinoacetate methyltransferase (GAMT) in the retina. Reverse transcription-polymerase chain reaction analysis revealed that L-arginine:glycine amidinotransferase and GAMT mRNAs were expressed in the retina and the Müller cell line, TR-MUL5. [14C]Creatine was detected after incubation of isolated rat retina or TR-MUL5 cells with [ 14C]glycine, L-arginine and L-methionine, suggesting creatine synthesis in Müller glia. Western blot analysis also revealed expression of GAMT protein in the rat retina and TR-MUL5 cells. Furthermore, confocal immunofluorescent microscopy of dual-labeled rat retinal sections demonstrated co-localization of GAMT with glutamine synthetase. Taken together, the results of the present study indicate creatine synthesis in Müller glia, implying an important role of creatine in energy metabolism in the retina.

AB - In high-energy metabolic tissues like the retina, creatine may play an important role in energy storage and in transmission of phosphate-bound energy substrates. To prove this, we investigated creatine synthesis in Müller glia. We also characterized the localization of the creatine synthetic enzyme, S-adenosyl-L-methionine:N-guanidinoacetate methyltransferase (GAMT) in the retina. Reverse transcription-polymerase chain reaction analysis revealed that L-arginine:glycine amidinotransferase and GAMT mRNAs were expressed in the retina and the Müller cell line, TR-MUL5. [14C]Creatine was detected after incubation of isolated rat retina or TR-MUL5 cells with [ 14C]glycine, L-arginine and L-methionine, suggesting creatine synthesis in Müller glia. Western blot analysis also revealed expression of GAMT protein in the rat retina and TR-MUL5 cells. Furthermore, confocal immunofluorescent microscopy of dual-labeled rat retinal sections demonstrated co-localization of GAMT with glutamine synthetase. Taken together, the results of the present study indicate creatine synthesis in Müller glia, implying an important role of creatine in energy metabolism in the retina.

KW - GAMT

KW - Glutamine synthetase

KW - Immunohistochemistry

KW - Müller cell line

KW - Rat retina

UR - http://www.scopus.com/inward/record.url?scp=27444435150&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27444435150&partnerID=8YFLogxK

U2 - 10.1002/glia.20222

DO - 10.1002/glia.20222

M3 - Article

C2 - 15892122

AN - SCOPUS:27444435150

VL - 52

SP - 47

EP - 52

JO - GLIA

JF - GLIA

SN - 0894-1491

IS - 1

ER -