TY - JOUR
T1 - Evidence for ethanol oxidation by Kupffer cells
AU - Nakamura, Yuji
AU - Yokoyama, Hirokazu
AU - Okamura, Yukishige
AU - Ohgo, Hideki
AU - Fukuda, Masahiko
AU - Horie, Yoshinori
AU - Kato, Shinzo
AU - Ishii, Hiromasa
PY - 1999/4
Y1 - 1999/4
N2 - Ethanol oxidation in Kupffer cells was investigated by measuring 14C- acetate formation from 14C-ethanol, and the role of aldehyde dehydrogenase 2 (ALDH2) in this process was also examined. Formation of 14C-acetate from 20 mM of 14C-ethanol was significantly increased in medium-containing Kupffer cells (9,003 ± 2,066 cpm/5 x 106 cells), compared with medium containing no cells (1,826 ± 46 cpm, p < 0.01), or containing acid-killed Kupffer cells (1,629 ± 210 cpm, p < 0.01). Ethanol formation was significantly attenuated when 20 and 200 μM cyanamide or 2 μM disulfiram were given. Reverse transcriptase-polymerase chain reaction demonstrated that Kupffer cells carry mRNA for ALDH2. These findings indicate that Kupffer cells can oxidize ethanol to acetate. ALDH2 may participate in this process, especially in the conversion of acetaldehyde to acetate.
AB - Ethanol oxidation in Kupffer cells was investigated by measuring 14C- acetate formation from 14C-ethanol, and the role of aldehyde dehydrogenase 2 (ALDH2) in this process was also examined. Formation of 14C-acetate from 20 mM of 14C-ethanol was significantly increased in medium-containing Kupffer cells (9,003 ± 2,066 cpm/5 x 106 cells), compared with medium containing no cells (1,826 ± 46 cpm, p < 0.01), or containing acid-killed Kupffer cells (1,629 ± 210 cpm, p < 0.01). Ethanol formation was significantly attenuated when 20 and 200 μM cyanamide or 2 μM disulfiram were given. Reverse transcriptase-polymerase chain reaction demonstrated that Kupffer cells carry mRNA for ALDH2. These findings indicate that Kupffer cells can oxidize ethanol to acetate. ALDH2 may participate in this process, especially in the conversion of acetaldehyde to acetate.
KW - ALDH2
KW - Cyanamide
KW - Disulfiram
KW - Ethanol Oxidation
KW - Kupffer Cell
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U2 - 10.1111/j.1530-0277.1999.tb04542.x
DO - 10.1111/j.1530-0277.1999.tb04542.x
M3 - Article
C2 - 10235287
AN - SCOPUS:0032929958
SN - 0145-6008
VL - 23
SP - 92S-95S
JO - Alcoholism: Clinical and Experimental Research
JF - Alcoholism: Clinical and Experimental Research
IS - 4 SUPPL.
ER -
