Evidence of direct interaction between Kupffer cells and colon cancer cells: An ultrastructural study of the co-culture

Y. Yonei, I. Kurose, D. Fukumura, Hidetsugu Saito, S. Miura, N. Tsukada, M. Oda, M. Tsuchiya

Research output: Contribution to journalArticle

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Abstract

A co-culture study of purified rat Kupffer cells and human colon cancer cells was performed, and the process of the tumor cell injury was observed under an inverted type fluorescence microscope loaded with propidium iodide, and also under an electron microscope. Ultrastructurally there was direct membrane-to-membrane interaction between Kupffer cells and colon cancer cells in time. The interaction occurred 1 h after start of the co-culture, and injured tumor cells were observed closely attached to pseudopodia of Kupffer cells at 6 h. The number of propidium iodide-positive tumor cells with damage increased in time. Pretreatment with N(G)-monomethyl-L-arginine reduced the number of injured tumor cells without preventing morphological interactions, but superoxide dismutase did not prevent the tumoricidal effect. Pretreatment with trypsin completely inhibited cell interaction and damage to tumor cells. In conclusion, the morphological interaction of Kupffer cells as a first step and the involvement of nitric oxide-derived free radicals as a second step seem to play a significant role in the host-defense mechanism.

Original languageEnglish
Pages (from-to)37-44
Number of pages8
JournalLiver
Volume14
Issue number1
Publication statusPublished - 1994

Fingerprint

Kupffer Cells
Coculture Techniques
Colonic Neoplasms
Propidium
Neoplasms
Pseudopodia
Membranes
Cell Communication
Trypsin
Superoxide Dismutase
Free Radicals
Arginine
Nitric Oxide
Fluorescence
Electrons
Wounds and Injuries

Keywords

  • cell culture
  • colon
  • colon cancer cell
  • Kupffer cell
  • liver

ASJC Scopus subject areas

  • Hepatology

Cite this

Yonei, Y., Kurose, I., Fukumura, D., Saito, H., Miura, S., Tsukada, N., ... Tsuchiya, M. (1994). Evidence of direct interaction between Kupffer cells and colon cancer cells: An ultrastructural study of the co-culture. Liver, 14(1), 37-44.

Evidence of direct interaction between Kupffer cells and colon cancer cells : An ultrastructural study of the co-culture. / Yonei, Y.; Kurose, I.; Fukumura, D.; Saito, Hidetsugu; Miura, S.; Tsukada, N.; Oda, M.; Tsuchiya, M.

In: Liver, Vol. 14, No. 1, 1994, p. 37-44.

Research output: Contribution to journalArticle

Yonei, Y, Kurose, I, Fukumura, D, Saito, H, Miura, S, Tsukada, N, Oda, M & Tsuchiya, M 1994, 'Evidence of direct interaction between Kupffer cells and colon cancer cells: An ultrastructural study of the co-culture', Liver, vol. 14, no. 1, pp. 37-44.
Yonei, Y. ; Kurose, I. ; Fukumura, D. ; Saito, Hidetsugu ; Miura, S. ; Tsukada, N. ; Oda, M. ; Tsuchiya, M. / Evidence of direct interaction between Kupffer cells and colon cancer cells : An ultrastructural study of the co-culture. In: Liver. 1994 ; Vol. 14, No. 1. pp. 37-44.
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