Evidence of Intercalation of trans-Diethylstilbestrol and Its Methyl Ether Derivatives in Multibilayers of Egg Phosphatidylcholine by High-Power Deuterium Nuclear Magnetic Resonance (2H-NMR) Spectroscopy

Taiko Oda, Yoshihiro Sato, Masahiko Kodam, Hazime Saito

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The mode of incorporation of 2 H-labeled trans-diethylstilbestrol (DES) (lb) and its methyl ether derivatives (2a, 2b, and 3) into multibilayers of egg phosphatidylcholine was analyzed by means of deuterium nuclear magnetic resonance. A clear distinction was found between DES or its methyl ether derivatives incorporated into lipid bilayers and those precipitated in the aqueous phase, by taking into account the extent of the motionally averaged quadrupole interaction. Thus, it was found that the relative proportion of these compounds incorporated into multibilayers decreased in the following order: DES (1b) > DES monomethyl ether (2a and 2b) > DES dimethyl ether (3). In addition, we demonstrated that the mode of intercalation in the multibilayers differs greatly among these compounds.

Original languageEnglish
Pages (from-to)2397-2399
Number of pages3
JournalChemical and Pharmaceutical Bulletin
Issue number9
Publication statusPublished - 1990 Jan 1



  • H-NMR
  • diethylstilbestrol
  • egg phosphatidylcholine
  • lipid bilayer
  • microtubule inhibitor
  • multibilayer
  • synthetic estrogen

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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