Evidence That MIF Plays a Role in the Development of Pigmentation Patterns in the Frog

Toshihiko Fukuzawa, Preminda Samaraweera, Francesco T. Mangano, John H. Law, Joseph T. Bagnara

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A ventrally localized melanization-inhibiting factor (MIF) may play an important role in the expression of dorsal-ventral pigment patterns of amphibians. In efforts to purify this putative MIF, ventral skin conditioned medium (VCM) from Rana forreri was partially fractionated and used to immunize mice. A monoclonal antibody that has the ability to block the activity of MIF was isolated, and an immunoaffinity matrix was prepared by cross-linking the antibody to protein G-Sepharose. The fraction of VCM that bound to the affinity matrix decreased the number of melanized cells in the Xenopus laevis neural tube explant assay, but did not reduce significantly the number of cells that emigrated. The monoclonal antibody was used for immunohistochemical studies on R. pipiens skin. Strong staining with the antibody was observed beneath the basement membrane, in mucous glands, and in the subcutaneous tissue of the ventral skin. A weak staining was also observed in the ground substances of both ventral and dorsal skin. These results confirm that a monoclonal antibody has been secured against at least one of the MIF constituents and that it is useful as a probe in detecting the distribution of MIF in tissues. The results of its use in this study support the hypothesis that MIF plays a role in the expression, development, and maintenance of the dorsal-ventral pigmentation patterns of frogs.

Original languageEnglish
Pages (from-to)148-158
Number of pages11
JournalDevelopmental Biology
Volume167
Issue number1
DOIs
Publication statusPublished - 1995 Jan
Externally publishedYes

Fingerprint

Pigmentation
Anura
Skin
Monoclonal Antibodies
Conditioned Culture Medium
Cell Count
Staining and Labeling
Ranidae
Neural Tube
Antibodies
Subcutaneous Tissue
Xenopus laevis
Thromboplastin
Amphibians
Basement Membrane
Sepharose
Maintenance
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Evidence That MIF Plays a Role in the Development of Pigmentation Patterns in the Frog. / Fukuzawa, Toshihiko; Samaraweera, Preminda; Mangano, Francesco T.; Law, John H.; Bagnara, Joseph T.

In: Developmental Biology, Vol. 167, No. 1, 01.1995, p. 148-158.

Research output: Contribution to journalArticle

Fukuzawa, Toshihiko ; Samaraweera, Preminda ; Mangano, Francesco T. ; Law, John H. ; Bagnara, Joseph T. / Evidence That MIF Plays a Role in the Development of Pigmentation Patterns in the Frog. In: Developmental Biology. 1995 ; Vol. 167, No. 1. pp. 148-158.
@article{f937c551f72f4e67abdf7028841a7f32,
title = "Evidence That MIF Plays a Role in the Development of Pigmentation Patterns in the Frog",
abstract = "A ventrally localized melanization-inhibiting factor (MIF) may play an important role in the expression of dorsal-ventral pigment patterns of amphibians. In efforts to purify this putative MIF, ventral skin conditioned medium (VCM) from Rana forreri was partially fractionated and used to immunize mice. A monoclonal antibody that has the ability to block the activity of MIF was isolated, and an immunoaffinity matrix was prepared by cross-linking the antibody to protein G-Sepharose. The fraction of VCM that bound to the affinity matrix decreased the number of melanized cells in the Xenopus laevis neural tube explant assay, but did not reduce significantly the number of cells that emigrated. The monoclonal antibody was used for immunohistochemical studies on R. pipiens skin. Strong staining with the antibody was observed beneath the basement membrane, in mucous glands, and in the subcutaneous tissue of the ventral skin. A weak staining was also observed in the ground substances of both ventral and dorsal skin. These results confirm that a monoclonal antibody has been secured against at least one of the MIF constituents and that it is useful as a probe in detecting the distribution of MIF in tissues. The results of its use in this study support the hypothesis that MIF plays a role in the expression, development, and maintenance of the dorsal-ventral pigmentation patterns of frogs.",
author = "Toshihiko Fukuzawa and Preminda Samaraweera and Mangano, {Francesco T.} and Law, {John H.} and Bagnara, {Joseph T.}",
year = "1995",
month = "1",
doi = "10.1006/dbio.1995.1013",
language = "English",
volume = "167",
pages = "148--158",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Evidence That MIF Plays a Role in the Development of Pigmentation Patterns in the Frog

AU - Fukuzawa, Toshihiko

AU - Samaraweera, Preminda

AU - Mangano, Francesco T.

AU - Law, John H.

AU - Bagnara, Joseph T.

PY - 1995/1

Y1 - 1995/1

N2 - A ventrally localized melanization-inhibiting factor (MIF) may play an important role in the expression of dorsal-ventral pigment patterns of amphibians. In efforts to purify this putative MIF, ventral skin conditioned medium (VCM) from Rana forreri was partially fractionated and used to immunize mice. A monoclonal antibody that has the ability to block the activity of MIF was isolated, and an immunoaffinity matrix was prepared by cross-linking the antibody to protein G-Sepharose. The fraction of VCM that bound to the affinity matrix decreased the number of melanized cells in the Xenopus laevis neural tube explant assay, but did not reduce significantly the number of cells that emigrated. The monoclonal antibody was used for immunohistochemical studies on R. pipiens skin. Strong staining with the antibody was observed beneath the basement membrane, in mucous glands, and in the subcutaneous tissue of the ventral skin. A weak staining was also observed in the ground substances of both ventral and dorsal skin. These results confirm that a monoclonal antibody has been secured against at least one of the MIF constituents and that it is useful as a probe in detecting the distribution of MIF in tissues. The results of its use in this study support the hypothesis that MIF plays a role in the expression, development, and maintenance of the dorsal-ventral pigmentation patterns of frogs.

AB - A ventrally localized melanization-inhibiting factor (MIF) may play an important role in the expression of dorsal-ventral pigment patterns of amphibians. In efforts to purify this putative MIF, ventral skin conditioned medium (VCM) from Rana forreri was partially fractionated and used to immunize mice. A monoclonal antibody that has the ability to block the activity of MIF was isolated, and an immunoaffinity matrix was prepared by cross-linking the antibody to protein G-Sepharose. The fraction of VCM that bound to the affinity matrix decreased the number of melanized cells in the Xenopus laevis neural tube explant assay, but did not reduce significantly the number of cells that emigrated. The monoclonal antibody was used for immunohistochemical studies on R. pipiens skin. Strong staining with the antibody was observed beneath the basement membrane, in mucous glands, and in the subcutaneous tissue of the ventral skin. A weak staining was also observed in the ground substances of both ventral and dorsal skin. These results confirm that a monoclonal antibody has been secured against at least one of the MIF constituents and that it is useful as a probe in detecting the distribution of MIF in tissues. The results of its use in this study support the hypothesis that MIF plays a role in the expression, development, and maintenance of the dorsal-ventral pigmentation patterns of frogs.

UR - http://www.scopus.com/inward/record.url?scp=0028883091&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028883091&partnerID=8YFLogxK

U2 - 10.1006/dbio.1995.1013

DO - 10.1006/dbio.1995.1013

M3 - Article

C2 - 7851638

AN - SCOPUS:0028883091

VL - 167

SP - 148

EP - 158

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -