Evolution of sequence recognition by restriction-modification enzymes

Selective pressure for specificity decrease

Akito Chinen, Yasuhiro Naito, Naofumi Handa, Ichizo Kobayashi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Several type II restriction-modification (RM) gene complexes kill host bacterial cells that have lost them, through attack on the chromosomal recognition sites of these cells. Two RM gene complexes recognizing the same sequence cannot simultaneously enjoy such stabilization through postsegregational host killing, because one will defend chromosomal sites from attack by the other. In the present work, we analyzed intrahost competition between two RM gene complexes when the recognition sequence of one was included in that of the other. When the EcoRII gene complex, recognizing 5'-CCWGG (W = A, T), is lost from the host, the SsoII gene complex, which recognizes 5'-CCNGG (N = A, T, G, C), will prevent host death by protecting CCWGG sites on the chromosome. However, when the SsoII (CCNGG) gene complex is lost, the EcoRII (CCWGG) gene complex will be unable to prevent host death through attack by SsoII on 5'-CCSGG (S = C, G) sites. These predictions were verified in our experiments, in which we analyzed plasmid maintenance, cell growth, cell shape, and chromosomal DNA. Our results demonstrate the presence of selective pressure for decrease in the specificity of recognition sequence of RM systems in the absence of invading DNA.

Original languageEnglish
Pages (from-to)1610-1619
Number of pages10
JournalMolecular Biology and Evolution
Volume17
Issue number11
Publication statusPublished - 2000

Fingerprint

Genes
enzyme
gene
Enzymes
enzymes
genes
DNA Restriction-Modification Enzymes
death
DNA
Cell Shape
restriction endonucleases
Cell growth
cells
Chromosomes
plasmid
chromosome
cell growth
plasmids
stabilization
Plasmids

Keywords

  • Molecular evolution
  • Restriction and modification
  • Sequence recognition

ASJC Scopus subject areas

  • Genetics
  • Biochemistry
  • Genetics(clinical)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Ecology, Evolution, Behavior and Systematics
  • Agricultural and Biological Sciences (miscellaneous)
  • Molecular Biology

Cite this

Evolution of sequence recognition by restriction-modification enzymes : Selective pressure for specificity decrease. / Chinen, Akito; Naito, Yasuhiro; Handa, Naofumi; Kobayashi, Ichizo.

In: Molecular Biology and Evolution, Vol. 17, No. 11, 2000, p. 1610-1619.

Research output: Contribution to journalArticle

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