Evolutionary dynamics of the human immunoglobulin κ locus and the germline repertoire of the Vκ genes

Kazuhiko Kawasaki, Shinsei Minoshima, Eriko Nakato, Kazunori Shibuya, Ai Shintani, Shuichi Asakawa, Takashi Sasaki, H. Gustav Klobeck, Gabriele Combriato, Hans G. Zachau, Nobuyoshi Shimizu

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

We have determined the entire nucleotide sequence of the human immunoglobulin κ locus, comprising a total of 1,010,706 nucleotides. The 76 Vκ genes found by a hybridization-based approach and their classification in 7 families were confirmed. A Vκ orphon located near the locus was also sequenced. In addition, we identified 55 novel Vκ relics and truncated pseudogenes, which establish 5 new families. Among these 132 Vκ genes, 46 have open reading frames. According to the databases and the literature, 32 unique Vκ genes and 5 identical gene pairs form VJ-joints, 27 unique genes and 4 gene pairs are transcribed, and 25 unique genes and 4 gene pairs produce functional proteins. The Vκ gene locus contains a 360-kb inverted duplication, which harbors 118 Vκ genes. A comparison of the duplicated Vκ genes suggests positive selection on the complementarity-determining regions of the duplicated genes by point mutations. The entire duplication unit was divided into 13 blocks, each of which has its distinct nucleotide sequence identity to its duplication counterpart (98.1-99.9%). An inversion-mediated mechanism is suggested to generate the high-homology blocks. Based on the homology blocks and the mutation rates, the inverted duplication is assumed to have taken place ∼ 5 million years ago. An orphon Vκ gene near the κ locus and a cluster of five Vκ orphons on chromosome 22 have no counterparts within the κ locus. This suggests possible mechanisms of the transposition of orphon Vκ genes.

Original languageEnglish
Pages (from-to)1017-1028
Number of pages12
JournalEuropean Journal of Immunology
Volume31
Issue number4
DOIs
Publication statusPublished - 2001

Fingerprint

Immunoglobulins
Genes
Complementarity Determining Regions
Chromosomes, Human, Pair 22
Pseudogenes
Mutation Rate
Point Mutation
Open Reading Frames
Nucleotides
Joints
Databases

Keywords

  • Evolution
  • Genome sequence
  • Germline repertoire
  • Immunoglobulin κ locus
  • Inverted duplication

ASJC Scopus subject areas

  • Immunology

Cite this

Evolutionary dynamics of the human immunoglobulin κ locus and the germline repertoire of the Vκ genes. / Kawasaki, Kazuhiko; Minoshima, Shinsei; Nakato, Eriko; Shibuya, Kazunori; Shintani, Ai; Asakawa, Shuichi; Sasaki, Takashi; Klobeck, H. Gustav; Combriato, Gabriele; Zachau, Hans G.; Shimizu, Nobuyoshi.

In: European Journal of Immunology, Vol. 31, No. 4, 2001, p. 1017-1028.

Research output: Contribution to journalArticle

Kawasaki, K, Minoshima, S, Nakato, E, Shibuya, K, Shintani, A, Asakawa, S, Sasaki, T, Klobeck, HG, Combriato, G, Zachau, HG & Shimizu, N 2001, 'Evolutionary dynamics of the human immunoglobulin κ locus and the germline repertoire of the Vκ genes', European Journal of Immunology, vol. 31, no. 4, pp. 1017-1028. https://doi.org/10.1002/1521-4141(200104)31:4<1017::AID-IMMU1017>3.0.CO;2-3
Kawasaki, Kazuhiko ; Minoshima, Shinsei ; Nakato, Eriko ; Shibuya, Kazunori ; Shintani, Ai ; Asakawa, Shuichi ; Sasaki, Takashi ; Klobeck, H. Gustav ; Combriato, Gabriele ; Zachau, Hans G. ; Shimizu, Nobuyoshi. / Evolutionary dynamics of the human immunoglobulin κ locus and the germline repertoire of the Vκ genes. In: European Journal of Immunology. 2001 ; Vol. 31, No. 4. pp. 1017-1028.
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AU - Sasaki, Takashi

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